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62.533 Aktuelle Fachpublikationen aus der Branche Biotechnologie
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01.07.2018 | Author(s): Ikrormi Rungsung, Muthukumaran Rajagopalan, Amutha Ramaswamy, Computational Biology and Chemistry, 2018
Publication date: Available online 30 June 2018 Source:Computational Biology and Chemistry Author(s): Ikrormi Rungsung, Muthukumaran Rajagopalan, Amutha Ramaswamy LKB1 protein is involved in the regulation of cell polarity by phosphorylating the AMPK under energetic stress conditions. ...
01.07.2018 | Sarah J. Fletcher; Vera P. Pisareva; Abdullah O. Khan; Andrew Tcherepanov; Neil V. Morgan; Andrey V. Pisarev, RNA, 2018
Platelets are anucleate and mostly ribosome-free cells within the bloodstream, derived from megakaryocytes within bone marrow and crucial for cessation of bleeding at sites of injury. Inherited thrombocytopenias are a group of disorders characterized by a low platelet count and are frequently ...
01.07.2018 | Gaëlle J.S. Talhouarne; Joseph G. Gall, RNA, 2018
We report that 7SL, the RNA component of the signal recognition particle (SRP), is an abundant noncoding RNA (ncRNA) in mature red blood cells (RBCs) of human, mouse, and the frog Xenopus. 7SL RNA in RBCs is not associated with the canonical proteins of the SRP. Instead, it coimmunoprecipitates ...
01.07.2018 | Malik Chaker-Margot, RNA, 2018
The eukaryotic ribosome is made of four intricately folded ribosomal RNAs and 79 proteins. During rapid growth, yeast cells produce an incredible 2000 ribosomes every minute. Ribosome assembly involves more than 200 trans-acting factors, intervening from the transcription of the preribosomal RNA ...
01.07.2018 | Adeline Pichard-Kostuch; Wenhua Zhang; Dominique Liger; Marie-Claire Daugeron; Juliette Létoquart; Ines Li de la Sie ..., RNA, 2018
N6-threonyl-carbamoyl adenosine (t6A) is a universal tRNA modification found at position 37, next to the anticodon, in almost all tRNAs decoding ANN codons (where N = A, U, G, or C). t6A stabilizes the codon–anticodon interaction and hence promotes translation fidelity. The first step of the ...
01.07.2018 | Vincent Boivin; Gabrielle Deschamps-Francoeur; Sonia Couture; Ryan M. Nottingham; Philia Bouchard-Bourelle; Alan M. ..., RNA, 2018
Comparing the abundance of one RNA molecule to another is crucial for understanding cellular functions but most sequencing techniques can target only specific subsets of RNA. In this study, we used a new fragmented ribodepleted TGIRT sequencing method that uses a thermostable group II intron ...
01.07.2018 | Sarah E. Fritz; Nazmul Haque; J. Robert Hogg, RNA, 2018
Cell-free systems are widely used to study mechanisms and regulation of translation, but the use of in vitro transcribed (IVT) mRNAs as translation substrates limits their efficiency and utility. Here, we present an approach for in vitro translation of messenger ribonucleoprotein (mRNP) complexes ...
01.07.2018 | Jessica S. Kurata; Ren-Jang Lin, RNA, 2018
MicroRNAs (miRNAs) are post-transcriptional gene regulators that play important roles in the control of cell fitness, differentiation, and development. The CRISPR-Cas9 gene-editing system is composed of the Cas9 nuclease in complex with a single guide RNA (sgRNA) and directs DNA cleavage at a ...
01.07.2018 | Kijun Kim; Trung Duc Nguyen; Shaohua Li; Tuan Anh Nguyen, RNA, 2018
The Microprocessor complex, consisting of an RNase III DROSHA and the DGCR8 dimer, cleaves primary microRNA transcripts (pri-miRNAs) to initiate microRNA (miRNA) maturation. Pri-miRNAs are stem–loop RNAs, and ~79% of them contain at least one of the three major and conserved RNA motifs, UG, UGU, ...
01.07.2018 | Mosharrof Mondal; Kody Mansfield; Alex Flynt, RNA, 2018
RNAi has revolutionized genetic research, and is being commercialized as an insect pest control technology. Mechanisms exploited for this purpose are antiviral and therefore rapidly evolving. Ideally, RNAi will also be used for noninsect pests; however, differences in RNAi biology make this ...
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