Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.
Autoren: |
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Aya Ito-Ishida; Hari Krishna Yamalanchili; Yingyao Shao; Steven A. Baker; Laura D. Heckman; Laura A. Lavery; Ji-yoen Kim; Laura M. Lombardi; Yaling Sun; Zhandong Liu; Huda Y. Zoghbi |
Journal: |
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Nature Neuroscience
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Band: |
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21 |
Ausgabe: |
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6 |
Jahrgang: |
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2018 |
Seiten: |
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794 |
DOI: |
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10.1038/s41593-018-0155-8
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Erscheinungsdatum: |
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25.05.2018 |