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Proteome of Bovine Mitochondria and Rod Outer Segment Disks: Commonalities and Differences

The retinal rod outer segment (OS) is a stack of disks surrounded by the plasma membrane, housing proteins related to phototransduction, as well as mitochondrial proteins involved in oxidative phosphorylation (OxPhos). This prompted us to compare the proteome of bovine OS disks and mitochondria to assess the significant top gene signatures of each sample. The two proteomes, obtained by LTQ-Orbitrap Velos mass spectrometry, were compared by statistical analyses. In total, 4139 proteins were identified, 2045 of which overlapping in the two sets. Nonhierarchical Spearman’s correlogram revealed that the groups were clearly discriminated. Partial least square discriminant plus support vector machine analysis identified the major discriminative proteins, implied in phototransduction and lipid metabolism, respectively. Gene Ontology analysis identified top gene signatures of the disk proteome, enriched in vesiculation, glycolysis, and OxPhos proteins. The tricarboxylic acid cycle and the electron transport prote...

Autoren:   Maurizio Bruschi; Andrea Petretto; Federico Caicci; Martina Bartolucci; Daniela Calzia; Laura Santucci; Lucia Manni; Luca A. Ramenghi; GianMarco Ghiggeri; Carlo E. Traverso; Giovanni Candiano; Isabella Panfoli
Journal:   Journal of Proteome Research
Jahrgang:   2018
DOI:   10.1021/acs.jproteome.7b00741
Erscheinungsdatum:   18.01.2018
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