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A reversible haploid mouse embryonic stem cell biobank resource for functional genomics

A reversible haploid mouse embryonic stem cell biobank resource for functional genomics

Nature 550, 7674 (2017). doi:10.1038/nature24027

Authors: Ulrich Elling, Reiner A. Wimmer, Andreas Leibbrandt, Thomas Burkard, Georg Michlits, Alexandra Leopoldi, Thomas Micheler, Dana Abdeen, Sergei Zhuk, Irene M. Aspalter, Cornelia Handl, Julia Liebergesell, Maria Hubmann, Anna-Maria Husa, Manuela Kinzer, Nicole Schuller, Ellen Wetzel, Nina van de Loo, Jorge Arturo Zepeda Martinez, David Estoppey, Ralph Riedl, Fengtang Yang, Beiyuan Fu, Thomas Dechat, Zoltán Ivics, Chukwuma A. Agu, Oliver Bell, Dieter Blaas, Holger Gerhardt, Dominic Hoepfner, Alexander Stark & Josef M. Penninger

The ability to directly uncover the contributions of genes to a given phenotype is fundamental for biology research. However, ostensibly homogeneous cell populations exhibit large clonal variance that can confound analyses and undermine reproducibility. Here we used genome-saturated mutagenesis to create a biobank of over 100,000 individual haploid mouse embryonic stem (mES) cell lines targeting 16,970 genes with genetically barcoded, conditional and reversible mutations. This Haplobank is, to our knowledge, the largest resource of hemi/homozygous mutant mES cells to date and is available to all researchers. Reversible mutagenesis overcomes clonal variance by permitting functional annotation of the genome directly in sister cells. We use the Haplobank in reverse genetic screens to investigate the temporal resolution of essential genes in mES cells, and to identify novel genes that control sprouting angiogenesis and lineage specification of blood vessels. Furthermore, a genome-wide forward screen with Haplobank identified PLA2G16 as a host factor that is required for cytotoxicity by rhinoviruses, which cause the common cold. Therefore, clones from the Haplobank combined with the use of reversible technologies enable high-throughput, reproducible, functional annotation of the genome.

Autoren:   Ulrich Elling; Reiner A. Wimmer; Andreas Leibbrandt; Thomas Burkard; Georg Michlits; Alexandra Leopoldi; Thomas Micheler; Dana Abdeen; Sergei Zhuk; Irene M. Aspalter; Cornelia Handl; Julia Liebergesell; Maria Hubmann; Anna-Maria Husa; Manuela Kinzer; Nicole Schuller; Ellen Wetzel; Nina van de Loo; Jorge Arturo Zepeda Martinez; David Estoppey; Ralph Riedl; Fengtang Yang; Beiyuan Fu; Thomas Dechat; Zoltán Ivics; Chukwuma A. Agu; Oliver Bell; Dieter Blaas; Holger Gerhardt; Dominic Hoepfner; Alexander Stark; Josef M. Penninger
Journal:   Nature
Band:   550
Ausgabe:   7674
Jahrgang:   2017
DOI:   10.1038/nature24027
Erscheinungsdatum:   27.09.2017
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