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Performance Evaluation of the Q Exactive HF-X for Shotgun Proteomics

Progress in proteomics is mainly driven by advances in mass spectrometric (MS) technologies. Here we benchmarked the performance of the latest MS instrument in the benchtop Orbitrap series, the Q Exactive HF-X, against its predecessor for proteomics applications. A new peak-picking algorithm, a brighter ion source, and optimized ion transfers enable productive MS/MS acquisition above 40 Hz at 7500 resolution. The hardware and software improvements collectively resulted in improved peptide and protein identifications across all comparable conditions, with an increase of up to 50 percent at short LC–MS gradients, yielding identification rates of more than 1000 unique peptides per minute. Alternatively, the Q Exactive HF-X is capable of achieving the same proteome coverage as its predecessor in approximately half the gradient time or at 10-fold lower sample loads. The Q Exactive HF-X also enables rapid phosphoproteomics with routine analysis of more than 5000 phosphopeptides with short single-shot 15 min LC–...

Autoren:   Christian D. Kelstrup; Dorte B. Bekker-Jensen; Tabiwang N. Arrey; Alexander Hogrebe; Alexander Harder; Jesper V. Olsen
Journal:   Journal of Proteome Research
Jahrgang:   2017
DOI:   10.1021/acs.jproteome.7b00602
Erscheinungsdatum:   20.12.2017
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