 |
|
|
|
 |
|
|
Malvern Applikationsnotizen
Method validation for laser diffraction measurements
When undertaking particle size measurements it is important to validate the analysis method, in order to determine its robustness and integrity. This application note outlines some of the important variables that should be assessed when considering the precision of laser diffraction measurements.
|
Wet method development for laser diffraction measurements
Particle size analysis using wet dispersion is the most widespread method for obtaining reproducible results using laser diffraction. This application note outlines the important parameters to be considered as part of method development when defining a standard operating procedure (SOP) for measuring wet dispersions using laser diffraction.
|
Controlling sample presentation during dry powder laser diffraction measurements
Sampling is one of the most important aspects of particle size analysis. The requirement for good sampling applies both to the way in which the test material is selected and how it is presented for measurement. The application notes examines the need for fine and coarse particles to travel through the measurement zone of a laser diffraction particle size analyzer at similar speeds in order to avoid a velocity bias, and how this is achieved.
|
Measurement of dairy and food emulsions using laser diffraction
The particle size of the fat droplets in dairy and other food emulsions is important in defining properties such as flavour release, mouth feel and emulsion stability. The wide dynamic range of the Mastersizer 2000 allows the measurement of both fine emulsion droplets and larger flocculated or coalesced particles. This application note describes the characterization of different milk products.
|
|
Zetasizer / Nano particles and Proteins
|
Molecular weight measurements with the Zetasizer Nano system
It is a common misconception that multi-angle light scattering is required in order to measure the absolute molecular weight of macromolecules. In this application note, the theoretical limits of single angle static light scattering are explored. Use of the Zetasizer Nano for measuring the molecular weight of small proteins and polymers is described.
|
Microemulsion characterization using dynamic light scattering
Dynamic light scattering is a non-invasive technique well suited to the study of colloidal dispersions such as microemulsions. This note illustrates how the suitability of microemulsions for the solubilisation of drugs can be determined by following the changes in size distributions and mean diameters with time. Experimental work uses the Zetasizer Nano system.
|
The relevance of particle size in protein processing
Useful biological materials often occur in a complex mixture of other materials and a sequence of separation and purification steps is usually required. This application note shows how photon correlation spectroscopy can be used to select optimum conditions for the microfiltration of protein solutions.
|
The relevance of zeta potential in protein processing
The surface properties of proteins, and especially the surface electrical properties, have an important influence on the performance of many separation processes. This application note describes the use of the Zetasizer system to measure electrophoretic mobility to characterize the surface electrical properties of proteins.
|
Process prediction in protein processing
Membrane ultrafiltration is a pressure-driven process that can separate macromolecules or colloidal particles from solvent or smaller solutes. A knowledge of the zeta potential of proteins can be valuable in identifying the pH and ionic strength that will give the fastest ultrafiltration of protein solutions. This application note describes the use of Zetasizer systems for this application.
|
Characterization of protein melting point
Dynamic light scattering has long been used as a protein characterization tool. This technical note describes how the sensitivity of DLS is sufficient to distinguish different oligomeric and quaternary protein states, making it suitable for monitoring the stability of protein structure to denaturing conditions.
|
Automated size and intensity trend measurements
There are advantages to automating particle size and scattering intensity trend measurements in many applications. Processes such as aggregation, sedimentation, solubilization and changes in molecular conformation can be followed by monitoring changes in the size and scattering intensity of a sample. This application note describes, with examples, trend measurement options for the Zetasizer Nano.
|
Image analysis and the importance of particle orientation
Image analysis is a powerful and increasingly widespread technique for the quantification of particle size and particle shape. Most shape descriptors used in these systems have been developed from the 2-D study of 3-D particles and any variance in particle orientation will lead to a diminution of data quality and reproducibility. This paper describes the technology used in leading systems from Malvern Instruments to ensure consistent orientation.
|
|
Weitere Informationen
Malvern Instruments GmbH
Herrenberg, Deutschland
Firmeninformation
News
Fachartikel
Produkte
|
|
|
|
|
|
Top
