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The most versatile second generation sequencing platform in the Life Science market
The Genome Sequencer FLX System from Roche Applied Science
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The Genome Sequencer FLX system from Roche is the improved successor of the GS 20 system, which was the first next generation sequencing system in the market. FLX stands for Flexibility and reflects the outstanding versatility of the system. Due to its improved combination of read length and throughput and its significantly improved sequence accuracy, a broad variety of break-through applications can be addressed at a higher quality. It is reasonable to assume that among other upcoming second-generation technologies providing only very short sequence reads (approximately 25 bases) the Genome Sequencer FLX System is the most versatile system. It offers the broadest range of applications for research fields such as cancer research, genetic diseases, infectious diseases, plant genomics or metagenomics, and many more.
The Genome Sequencer FLX features and resulting benefits include
- Average read length of 200--300 bases (compared to 100 with GS 20), depending of the application and the organism. Read length is one of the most important key. The longer the read length, the fewer gaps will remain in consensus sequences of whole genome sequencing projects, the more accurate the identification of highly variable alleles will be, and the more information on haplotypes will be gained. Longer read length also facilitates to allocate functions to EST sequences derived from the transcriptome.
- Single read accuracy of more than 99.5%, substitution errors are exceedingly rare
- Increased throughput leading to faster, more convenient and less costly data generation: 200 Mb per day
- Improved reagents concept (On-board mixing of sequencing reagents, improved fluidics reducing run time and reagent consumption, improved reagent shelf life, faster rapid thaw protocols for reagents (2 hours at RT), only one preventive maintenance per year due to automated, tube calibration, even greater robustness)
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With the Genome Sequencer FLX System, 454 Life Science and Roche Applied Science have introduced their "second- generation Genome Sequencer System".
For many applications in the various research fields, read lengths >100 bases -- as offered by the GS FLX - are required to uncover the comprehensive biological information encoded in DNA sequences, such as SNPs in both exonic or intronic sequences, structural variants, or very important small deletions in the range of 3--500 bases. An incomplete picture of the real situation is often seen when smaller read lengths are used. This results in misleading and expensive downstream analysis.
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