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Varian, Inc. Introduces Its First BioStandard Used to Validate DHPLC Systems

10 Apr 2002 - PALO ALTO, Calif., - Varian, Inc. announced that the company has begun manufacturing biomolecular standards for the first time, as part of its BioSolutions(TM) line of products. The new DYS271 DHPLC Standard is ready-made and designed for easy, routine validation of denaturing high performance liquid chromatography (DHPLC) systems' operation. Geneticists use DHPLC to discover and screen for changes in DNA sequences, including single nucleotide polymorphisms (SNPs). DHPLC is one of the most popular techniques for the discovery of novel mutations in DNA and for screening of clinical samples.
 
A nucleotide substitution mutation within the DYS271 sequence tagged site (reported by Seielstad, et al. 1994) was originally used by DHPLC inventors Peter Oefner and Peter Underhill in early proof of principle experiments at Stanford University and has since become a valuable tool to evaluate equipment performance. DYS271 DHPLC Standard is a 209 base pair DNA fragment with a well-characterized single nucleotide polymorphism (SNP) at position 168. The Y chromosome derived standard is amplified by PCR out of a plasmid vector using gene specific primers. The standard is used to validate Varian's Helix(TM) System and other DHPLC instruments.
 
The DYS271 DHPLC Standard is lyophilized, or dried-down, and is stable for longer periods of time than already hydrated standards. It is manufactured by Varian under an ISO9001 certified process. DYS271 is currently available for shipment.