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Nile red
| Nile red |
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| IUPAC name |
Nile red |
| Other names |
Nile blue oxazone |
| Identifiers |
| CAS number |
7385-67-3 |
| PubChem |
65182 |
| SMILES |
CCN(CC)C1=CC2=C(C=C1)N= C3C4=CC=CC=C4C(=O)C=C3O2 |
| Properties |
| Molecular formula |
C20H18N2O2 |
| Molar mass |
318.369 g/mol |
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references |
Nile red (also known as Nile blue oxazone) is a lipophilic stain. It is produced by boiling a solution of Nile blue with sulfuric acid. As can be seen from the structural formulae, this process replaces an amino group with a carbonyl group. Nile red stains intracellular lipid droplets red. Nile red is also intensely fluorescent, with a strong yellow-gold emission when in a lipid-rich environment.
Since the reaction to generate Nile red does not usually completely exhaust the supply of Nile blue, additional separation steps are required if pure Nile red is needed.
External links
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This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Nile_red". A list of authors is available in Wikipedia.
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