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Hygromycin B
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Hygromycin B
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| Systematic (IUPAC) name |
| O-6-Amino-6-deoxy-L-glycero- D-galacto- heptopyranosylidene- (1-2-3)-O-beta-D-talopyranosyl (1-5) -2-deoxy-N3-methyl-D-streptamine |
| Identifiers |
| CAS number |
31282-04-9 |
| ATC code |
? |
| PubChem |
35766 |
| Chemical data |
| Formula |
C20H37N3O13 |
| Mol. mass |
527.53 g/mol (563.5 with HCl) |
| Pharmacokinetic data |
| Bioavailability |
? |
| Metabolism |
? |
| Half life |
? |
| Excretion |
? |
| Therapeutic considerations |
| Pregnancy cat. |
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| Legal status |
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| Routes |
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Hygromycin B, is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis[1].
History
Hygromycin B was originally developed in the 1950's for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: Hygromix). Hygromycin B is produced by Streptomyces hygroscopicus, a bacterium isolated in 1953 from a soil sample. Resistance genes were discovered in the early 1980's.[2][3]
Use in research
In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation.[4] Since the discovery of hygromycin-resistance genes, hygromycin B has become a standard selection antibiotic in gene transfer experiments in many prokaryotic and eukaryotic cells.
References
- ^ McGuire, Pettinger (1953), " ", Antibiot. Chemother. 3: 1268-1278
- ^ Davies, Gritz (1983), " ", Gene 25: 179-88
- ^ Burgett, Kaster (1983), " ", Nucleic Acids Res. 11: 6895-911
- ^ Rao RN, Allen NE, Hobbs JN, Alborn WE, Kirst HA, Paschal JW (1983). "Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli". Antimicrobial Agents and Chemotherapy 24 (5): 689-95. PMID 6318654.
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This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Hygromycin_B". A list of authors is available in Wikipedia.
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