Fixed Dose Procedure (FDP), proposed in 1984, is an assess of a substance's acute oral toxicity using fewer animals with less suffering than the older LD50 test developed in 1927.
FDP uses about 10 to 20 animals to find the dose producing toxicity signs but not death, and from there predicts the lethal dose. LD50 ("lethal dose 50%") uses about 60 to 80 animals to find a dose killing 50% of animals within a given time. FDP sometimes requires retesting using slightly higher or lower doses.
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