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Growth of Campylobacter using BMG LABTECH’s FLUOstar Omega equipped with ACU
Comparison of growth in a test tube incubator to 96 well plates in a FLUOstar Omega with ACU
03-11-2011
Growth of Microaerophiles using Campylobacter as a model Comparison of growth in a traditional environmentally controlled test tube incubator to 96 well plates in a FLUOstar Omega with ACU Independent Oxygen and Carbon Dioxide regulation allows for optimal growth conditions for different ...
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega
03-11-2011
Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...
Characterizing Biotherapeutics with HPLC
Smart Solutions for large Molecules
24-11-2010
Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...
Agrisera Western Blot recommendations, troubleshooting and protocol
24-11-2010
Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number ...
Analysis of water soluble polymers using linear size exclusion HPLC columns and a semi-micro SEC system
16-11-2010
In recent years water-soluble polymers are gaining more and more interest in different applications. The molecular weight distribution of polymers is usually characterized by size exclusion chromatography (SEC) coupled with refractive index, viscometric or laser light scattering detection. ...
Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization
22-09-2010
High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar ...
Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
23-06-2010
Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...
Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader
16-06-2010
Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...
The New MagNA Pure 96 DNA and Viral NA Kits: Maximal Speed Combined with High Recovery
18-01-2010
Introduction The MagNA Pure 96 System is a brand-new system recently introduced to the market: it is a fully automated, ultra-fast system for high-throughput isolation of high- quality nucleic acids from various sample types that can handle sample volumes of up to 1 ml. The main features ...
Successful Isolation of a Proliferating Cell Population with Stem Cell-like Properties from Rat Pancreas
Using Liberase TL Research Grade
04-01-2010
In this study, the performance of Liberase TL Research Grade for the isolation of early progenitor cells from rat pancreatic tissue was analyzed. Tissue digestion with Liberase TL Research Grade resulted in high yields of isolated cells. Markers for the three germ layers (ecto-, endo- and ...
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