12-10-2010

#img6106# In the Life Sciences and Bio-medical Research, the characterization of chemical compounds within cells or tissues is one of the most important and difficult tasks for researchers to perform. The development of pharmaceuticals such as drug delivery coatings or capsules also requires ...

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Comparison of 2 methods

17-03-2010

In the pharmaceutical and food industry, colorimetrically stained samples like gels stained with Coomassie® are an important method for quality control. The acquisition of these samples for documentation and quantification purposes can be realised in two different ways. In addition to ...

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25-01-2010

Introduction Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, ...

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18-01-2010

Introduction Advancements in high-throughput gene expression analysis have set a new demand for automated isolation of cellular RNA from high sample numbers in parallel. The availability of the innovative MagNA Pure 96 System together with dedicated kits, protocols, and consumables sets a ...

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11-01-2010

In this study, the xCELLigence System was used to determine and characterize cell death after transfection of the bax gene into HeLa cells using three different commercially available transfection reagents. With the workflow presented here, it was possible not only to evaluate and compare the ...

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21-12-2009

Introduction High Resolution Melting (HRM) is a novel and promising screening technique used for mutation analysis and detection. Its application prevents the need to sequence all the exons of a gene when looking for novel mutations. The method is therefore time- and cost-effective for the ...

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14-12-2009

Introduction Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to neuroblastoma cells exposed ...

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Gene expression profiling of the human genome on the LightCycler® 480 System has never been so easy.

14-12-2009

Function tested qPCR assays for human targets are now only a few clicks away, with the new RealTime ready Configurator. This free online assay selection and shopping tool can be used to order single assays (for up to 300 20-µl reactions) or to configure customized panels on LightCycler® 480 ...

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A Cross-Platform Comparison

09-12-2009

Introduction During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public ...

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30-11-2009

Introduction The power of the Genome Sequencer FLX System and its utility for sequencing amplicons derive from the ability to sequence from single molecules within a mixture of molecules. In this process, each amplicon molecule, also known as a PCR product, is sequenced individually, ...

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