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10 Current white paper about the topic inhibitor
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Assessing Microbial Metabolism Using A Simple ‘Mix & Measure’ Oxygen Consumption Assay
25-05-2011
High throughput analysis of microbial O2 consumption to assess microbial metabolism MitoExpress®, water soluble “mix and measure” O2 sensing probes Multiplexing with FLUOstar Omega to measure both growth (OD 600) and O2 consumption Introduction MitoExpress® (Luxcell Biosciences) is a ...
Altered Expression of Broad Range of Protease Genes in SH-SY5Y Neuroblastoma Cells after Bortezomib Exposure
14-12-2009
Introduction Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to neuroblastoma cells exposed ...
Amplicon Sequencing with the GS FLX Titanium Series: High Sensitivity Targeted Resequencing
30-11-2009
Introduction The power of the Genome Sequencer FLX System and its utility for sequencing amplicons derive from the ability to sequence from single molecules within a mixture of molecules. In this process, each amplicon molecule, also known as a PCR product, is sequenced individually, ...
Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
25-11-2009
Pseudomonas elastase activity monitored using Abz-Nba internally quenched substrate Km and Vmax calculated using MARS Data Analysis Software Michaelis Menten kinetics determined for a library of 160 elastase inhibitors Introduction Pseudomonas elastase (pseudolysin, LasB) is a ...
Trichostatin A Treatment of Cells Transfected with FuGENE® HD Transfection Reagent
14-05-2009
Introduction Treatment of eukaryotic cells with trichostatin A (TSA) – a broad-spectrum inhibitor of histone deacetylation – is frequently used in studies of epigenetic regulation of gene expression. To be able to investigate the effects of TSA on promoter activity in reporter gene assays it ...
Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR
04-06-2008
New Enzyme Class Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack of accuracy leads to a significant number of base exchanges or frameshifts which are further ...
Efficient Transfection of VeroE6 Cells with DNA and RNA for Luciferase Reporter Assays Using FuGENE® HD Transfection Reagent
28-05-2008
Introduction The interferon (IFN) system of mammalian cells is the first line of defense against viral infections. Following infection, danger signals such as double-stranded RNA or single-stranded RNA with an unmodified 5´ triphosphate end (e.g., VSV-RNA) are recognized by membrane-bound ...
Label-Free and Dynamic Monitoring of Cell-Based Assays
27-05-2008
Introduction The xCELLigence system from Roche Applied Science, originally invented by the US company ACEA Biosciences, allows for label-free and dynamic monitoring of cell-based assays. The xCELLigence system utilizes impedance readout to non-invasively quantify cellular status in real-time ...
HelpCorner - Nucleic Acid Isolation and Purification
20-05-2008
This article deals with selected frequently asked questions (FAQs) on nucleic acid isolation and purification. Isolation of RNA FAQ: Which High Pure kit do you offer for the isolation of total RNA from formalin-fixed, paraffin-embedded tissue? HelpCorner: For the isolation of total RNA from ...
Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
29-08-2007
MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18–25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs ...
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