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58 Current white paper about the topic genes

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Quantification of BRCA1 Expression Level Using Standard RT-PCR Reagents - a Sensitive Method for the Detection of Low Amounts of Transcripts

17-Aug-2007

Mutations in the breast cancer susceptibility gene, BRCA1, occur in the majority of families with multiple members affected with breast or ovarian cancer. Women who inherit a BRCA1 mutation have 40%-80% risk of developing breast cancer or ovarian can

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Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies

16-Aug-2007

The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of

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Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent

15-Aug-2007

Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient

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LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

09-Aug-2007

The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, s

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siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

08-Aug-2007

Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentia

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Detection of Avian H5N1 Virus with the LightCycler® Instrument

07-Aug-2007

The avian H5N1 influenza virus has killed thousands of birds and caused several fatal cases in humans in Asia and Turkey. A fast and reliable test to identify the virus is urgently required for an effective epedemiological control, and associated med

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LightCycler® 480 Instrument: High-throughput Genotyping for TLR-8 SNPs

03-Aug-2007

Determining frequencies of single nucleotide polymorphisms (SNPs) in large populations is of great interest in different fields of molecular medicine, including pharmacogenetics, risk profiling, and candidate gene testing. Several SNPs within innate

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Improved Performance of the LightCycler®

FastStart DNA MasterPLUS SYBR Green I Kit in Highly Sensitive Transcript Quantification

11-Jun-2007

Real-time reverse transcription-PCR (RT-PCR) is con­sidered the method of choice for any experiments requiring­ sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular

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