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32 Current white paper about the topic genesrss
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An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential e
The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) . Combined with Thermus
Transfected cell microarray is a versatile, efficient, and cost-reducing technology [1,3,4] that can contribute to further understanding of gene functions and regulatory mechanisms governing gene expression. We have optimized the protocol of transfec
Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggest
Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis of biological functions. Such screens commonly res
Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA m
Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are gener
Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific environment.
RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the past years, more than 500 novel genes have been ide
Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-fo