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58 Current white paper about the topic genes

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Altered Expression of Broad Range of Protease Genes in SH-SY5Y Neuroblastoma Cells after Bortezomib Exposure

14-Dec-2009

Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to

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Acquired Copy Number Alterations in Adult Acute Myeloid Leukemia Genomes

30-Nov-2009

To complement cytogenetic studies and to identify genes altered in AML genomes, Walter and co-workers performed a genome-wide copy number analysis with paired normal and tumor DNA obtained from 86 adult subjects with de novo AML using 1.85 million fe

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Amplicon Sequencing with the GS FLX Titanium Series: High Sensitivity Targeted Resequencing

30-Nov-2009

Amplicon sequencing with the Genome Sequencer FLX System has already enabled a wide variety of scientific discoveries, particularly in clinical research. The introduction of the GS FLX Titanium Chemistry and the release of the associated amplicon seq

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-Sep-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-Sep-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential e

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Efficient Reverse Transfection of Both siRNA and Expression Plasmids on Transfected Cell Microarrays Using X-tremeGENE siRNA Transfection Reagent

24-Aug-2009

Transfected cell microarray is a versatile, efficient, and cost-reducing technology [1,3,4] that can contribute to further understanding of gene functions and regulatory mechanisms governing gene expression. We have optimized the protocol of transfec

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Frequent Switching of Polycomb Repressive Marks and DNA Hypermethylation in the PC3 Prostate Cancer Cell Line

17-Aug-2009

A key observation in these presented results was the magnitude of hypermethylation in PC3 cells, which occurred at almost all of the inactive CpG island genes. A second key observation was that when PRC marks were depleted, a PC3 hypermethylation was

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Rare Variants of IFIH1 Protect Against Type-1 Diabetes

10-Aug-2009

Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggest

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qPCR Identification of Genes Involved in Apoptosis and Cell Cycle Regulation

10-Jun-2009

Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis of biological functions. Such screens commonly res

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Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System

08-Jun-2009

Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA m

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