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45 Current white paper about the topic genes

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-Sep-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus

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Efficient Reverse Transfection of Both siRNA and Expression Plasmids on Transfected Cell Microarrays Using X-tremeGENE siRNA Transfection Reagent

24-Aug-2009

Transfected cell microarray is a versatile, efficient, and cost-reducing technology [1,3,4] that can contribute to further understanding of gene functions and regulatory mechanisms governing gene expression. We have optimized the protocol of transfec

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Rare Variants of IFIH1 Protect Against Type-1 Diabetes

10-Aug-2009

Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggest

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qPCR Identification of Genes Involved in Apoptosis and Cell Cycle Regulation

10-Jun-2009

Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis of biological functions. Such screens commonly res

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Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System

08-Jun-2009

Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA m

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Outstanding Microarray Experimental Results Using FuGENE® HD Transfection Reagent

03-Jun-2009

Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are gener

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Characterizing Environments: Recent Discoveries Enabled by Metagenomic Analysis with the Genome Sequencer FLX System

08-May-2009

Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific environment.

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miRNA Extraction and Profiling on FFPE Samples

08-Sep-2008

RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the past years, more than 500 novel genes have been ide

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Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent

22-Aug-2008

Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-fo

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High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System

03-Jun-2008

During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been

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