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New Products for Cell Culture Analysis from Roche Innovatis
31-08-2009
The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...
Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent
22-08-2008
Introduction Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study ...
Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent
22-05-2008
Introduction The experimental method of introducing foreign molecules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of ...
Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers
29-02-2008
Promega’s DLR assay has been validated on the LUMIstar Omega, FLUOstar Omega, and POLARstar Omega Two onboard reagent injectors with simultaneous measurement allow for instantaneous detection of Firefly and Renilla luciferase Introduction The Dual-Luciferase® Reporter Assay or DLR is ...
Efficient Transfection of 293T Cell Line with Lentiviral Vectors Using FuGENE® HD Transfection Reagent
03-10-2007
Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T ...
Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
26-09-2007
Introduction Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are ...
Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent
15-08-2007
Introduction Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient transfection, molecules do not ...
Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses
14-08-2007
IntroductionMany viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection [1, 2]. Transfection of viral genes into cultures of mammalian cells is an important research tool ...
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