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34 Current white paper about the topic fluorescence

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HelpCorner - xCELLigence System

25-01-2010

Automated monitoring and data collection contribute to significant time savings when using the xCELLigence System, with only a few minutes of extra setup time. For example, in a 96-well plate, 32 different conditions can be tested in triplicate; the wells need to be seeded with cells, monitored, ...

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Functional Analysis of Side Effects of Transfection Reagents in the Context of Bax-induced Apoptosis

11-01-2010

FuGENE® HD Transfection Reagent is well-suited for studying the impact of apoptosis-inducing proteins such as bax. Transfection with FuGENE® HD Transfection Reagent produced only minimal cytotoxic side effects while maintaining a high transfection efficiency. In contrast, cytotoxicity, and ...

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New Products for Cell Culture Analysis from Roche Innovatis

31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...

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HelpCorner - Transfection

31-08-2009

Transfection is the delivery of DNA, RNA, proteins, and macromelecules into eukaryotic cells. The goals for transfection include the study of gene function and regulation as well as protein expression and function. It is also used for gene silencing and protein production. According to the ...

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Efficient Reverse Transfection of Both siRNA and Expression Plasmids on Transfected Cell Microarrays Using X-tremeGENE siRNA Transfection Reagent

24-08-2009

Transfected cell microarray is a versatile, efficient, and cost-reducing technology [1,3,4] that can contribute to further understanding of gene functions and regulatory mechanisms governing gene expression. We have optimized the protocol of transfected cell microarrays using X-tremeGENE siRNA ...

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qPCR Identification of Genes Involved in Apoptosis and Cell Cycle Regulation

10-06-2009

Systematic high-throughput functional genomic screens with the targeted silencing of genes by the introduction of small interfering RNA (siRNA) have emerged as an extremely powerful tool for analysis of biological functions. Such screens commonly result in a huge amount of "hits", genes that ...

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Dynamics of Kinases Involved in Cell Growth Using the xCELLigence System

08-06-2009

Studies investigating endogenous cellular regulatory mechanisms have substantially pushed the application of high-throughput screens. RNA interference has developed into an extremely powerful tool for mediating the loss-of-function of specific mRNA molecules and preventing their translation into ...

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Outstanding Microarray Experimental Results Using FuGENE® HD Transfection Reagent

03-06-2009

Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are generally irrelevant, unless the changes relate ...

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Trichostatin A Treatment of Cells Transfected with FuGENE® HD Transfection Reagent

14-05-2009

Treatment of eukaryotic cells with trichostatin A (TSA) - a broad-spectrum inhibitor of histone deacetylation - is frequently used in studies of epigenetic regulation of gene expression. To be able to investigate the effects of TSA on promoter activity in reporter gene assays it is necessary to ...

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