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7 Current white paper about the topic fluorescencerss
The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentratio
Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-fo
The experimental method of introducing foreign molecules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introdu
The Dual-Luciferase® Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla. The Firefly reaction is initiated, followed by its quenc
Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfe
Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfect
Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient