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8 Current white paper about the topic fluorescence

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New Products for Cell Culture Analysis from Roche Innovatis

31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More information about Roche Innovatis products will appear in future issues of Biochemica...

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Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent

22-08-2008

Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study the signaling network involved in odontogenesis, we co-cultured primary human fetal dental epithelial cells and dental pulp cells to reconstitute the reciprocal signaling....

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Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent

22-05-2008

The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid DNA, RNA, siRNA, and miRNA) into eukaryotic cells [1,2]. However, the optimal condition for incorporation depends on the type of transfection reagent and the cells being used, resulting in variable transfection efficiency and the extent of cell damage caused by transfection....

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Dual Luciferase Reporter (DLR) Assay Certification on the Omega Series of Readers

29-02-2008

The Dual-Luciferase® Reporter Assay or DLR is widely used to study gene transcription and regulation. The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction....

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Efficient Transfection of 293T Cell Line with Lentiviral Vectors Using FuGENE® HD Transfection Reagent

03-10-2007

Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T cells with lentiviral vectors with high efficiency....

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Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

26-09-2007

Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency....

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Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent

15-08-2007

Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient transfection, molecules do not integrate into the host cell genome and are only expressed for approximately 24 hours - 96 hours post-transfection....

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Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses

14-08-2007

Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways....

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