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7 Current white paper about the topic fluorescencerss
The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen ...
A Cross-Platform Comparison
During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in
An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential e
The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) . Combined with Thermus
When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed up concentration rates, saving researchers time, ...
The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now ...
Determining frequencies of single nucleotide polymorphisms (SNPs) in large populations is of great interest in different fields of molecular medicine, including pharmacogenetics, risk profiling, and candidate gene testing. Several SNPs within innate