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7 Current white paper about the topic fluorescence

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader

16-06-2010

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen a number of samples with the help of the FLUOstar OPTIMA multidetection microplate reader from BMG LABTECH...

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LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels

A Cross-Platform Comparison

09-12-2009

During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public research, with major impact on the fields of drug discovery, molecular medicine and personalized health care...

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-09-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) protein in an isothermal amplification procedure, the technique enables suppression of mis-amplification of highly related sequences (e.g., a wild-type sequence versus an SNP variant), minimizing background amplification. The Taq MutS protein binds to mis-matched nucleotides in erroneously primed dsDNA and prevents further amplification by blocking the dissociation of DNA duplexes having a mis-match by DNA polymerases having strand displacement activity. SmartAmp-2 assays have been used for detection of various SNPs and mutations, e.g., in ALDH2 and EGFR genes [1,2]. In the study presented here, we investigated whether this assay principle could be adapted for detection of microsatellite polymorphisms showing copy number differences in...

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-09-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where individual samples are screened against many thousands of target genes. According to its superior sensitivity and accuracy, qPCR is a well-established gold standard for precise profiling of gene expression levels or genotyping of known SNPs...

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A Rapid, Sample-Safe, Evaporative Technique for Preparation of DNA and Oligonucleotide Samples

02-03-2009

When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed up concentration rates, saving researchers time, and also eliminate potential sources of damage to samples...

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Novel Methods for High-Performance Melting Curve Analysis Using the LightCycler® 480 System

23-08-2007

The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now facilitates advanced applications in the emerging field of amplicon scanning for new mutations at high resolution....

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LightCycler® 480 Instrument: High-throughput Genotyping for TLR-8 SNPs

03-08-2007

Determining frequencies of single nucleotide polymorphisms (SNPs) in large populations is of great interest in different fields of molecular medicine, including pharmacogenetics, risk profiling, and candidate gene testing. Several SNPs within innate immunity genes have been shown to play a role in complex human diseases. Among them, SNPs within Toll-like Receptor (TLR) genes are known to influence susceptibility to infectious diseases....

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