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7 Current white paper about the topic fluorescence

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader

16-06-2010

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen ...

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LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels

A Cross-Platform Comparison

09-12-2009

During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public research, with major ...

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-09-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where ...

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-09-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) protein in an ...

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A Rapid, Sample-Safe, Evaporative Technique for Preparation of DNA and Oligonucleotide Samples

02-03-2009

When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed up concentration rates, saving researchers time, ...

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Novel Methods for High-Performance Melting Curve Analysis Using the LightCycler® 480 System

23-08-2007

The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now ...

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LightCycler® 480 Instrument: High-throughput Genotyping for TLR-8 SNPs

03-08-2007

Determining frequencies of single nucleotide polymorphisms (SNPs) in large populations is of great interest in different fields of molecular medicine, including pharmacogenetics, risk profiling, and candidate gene testing. Several SNPs within innate immunity genes have been shown to play a role ...

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