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7 Current white paper about the topic fluorescence
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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader
16-06-2010
Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...
LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels
A Cross-Platform Comparison
09-12-2009
Introduction During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public ...
Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument
07-09-2009
Introduction The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) ...
High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System
07-09-2009
Introduction An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional ...
A Rapid, Sample-Safe, Evaporative Technique for Preparation of DNA and Oligonucleotide Samples
02-03-2009
The difficulties of concentration of oligonucleotides and especially tagged oligonucleotides are well documented in the literature1. Adverse conditions can damage the sample, and in some cases totally degrade it. When sourcing a concentration method for their microtitre plates containing ...
Novel Methods for High-Performance Melting Curve Analysis Using the LightCycler® 480 System
23-08-2007
The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now ...
LightCycler® 480 Instrument: High-throughput Genotyping for TLR-8 SNPs
03-08-2007
#img4139# Introduction Determining frequencies of single nucleotide polymorphisms (SNPs) in large populations is of great interest in different fields of molecular medicine, including pharmacogenetics, risk profiling, and candidate gene testing [1]. Several SNPs within innate immunity genes ...
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