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14 Current white paper about the topic fluorescence

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader

16-06-2010

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen ...

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Major Histocompatibility Complex Genotyping with Massively Parallel Pyrosequencing

04-01-2010

Macaques provide essential preclinical models for infectious disease, vaccine, biodefense, and transplantation research. Unfortunately, they also have the most complex MHC genetics of any primate species described to date, and existing methods for MHC typing are simply inadequate. As shown here, ...

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LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels

A Cross-Platform Comparison

09-12-2009

During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public research, with major ...

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-09-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where ...

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-09-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) protein in an ...

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Rare Variants of IFIH1 Protect Against Type-1 Diabetes

10-08-2009

Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggests that variants that disrupt IFIH1 function in ...

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A Rapid, Sample-Safe, Evaporative Technique for Preparation of DNA and Oligonucleotide Samples

02-03-2009

When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed up concentration rates, saving researchers time, ...

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Microsatellite Analysis of Grapevine Varieties by High-Resolution Amplicon Melting Analysis

30-05-2008

Certification of grapevine scion and rootstock varieties is becoming an increasingly important issue in viticulture. Many rootstocks are derived from the same parentage - for example 5C, SO4, 5BB, 125AA, and 420A Mgt are all used in New Zealand and are all derived from crosses between Vitis ...

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Genotypes in Smokers: Correlations with Smoking Behavior

12-09-2007

Smoking behavior is influenced by both genetic and environmental factors. Moreover, smoking initiation and smoking persistence have a heritability of at least 50%. A large body of work has been dedicated to associating multiple genetic markers of neurobiological pathways previously linked to ...

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Experimental Workflow for Fast and Accurate Genotyping of Scrapie-resistant/sensitive Sheep

05-09-2007

Several scientific studies support the claim that natural scrapie is associated with polymorphisms at three codons within the sheep prion protein (PrP) gene. Genotyping of these codons (codon 136 [alanine, valine], 154 [arginine, histidine], and 171 [glutamine, arginine, histidine]) might enable ...

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