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42 Current white paper about the topic fluorescencerss
Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega
The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured in 96-well format
On the RealTime ready Configurator, we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL). The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing modified bases in ...
Smart Solutions for large Molecules
As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmace
The alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells. Active cells are able to convert resazurin into the resorufin. Resazurin is blue and non-fluorescent whereas resorufin is red and highly fluorescent; therefore a fluorescent and a ...
The POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in 96-well format. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid ...
The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen ...
Although congenital heart malformations are the most common birth defects in humans, the underlying pathomechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, NKX2-5) have been ...
A Cross-Platform Comparison
During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in
Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic ...
In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species (ROS). ROS are necessary intermediates in the human body, but they are also involved in the aging process and in the development of ...