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8 Current white paper about the topic fluorescence

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RealTime ready RT-qPCR Assay Development and Qualification

01-06-2011

On the RealTime ready Configurator, we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL). The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing modified bases in order to stabilize the binding and thus ensure high flexibility together with good amplicon specificity...

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LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels

A Cross-Platform Comparison

09-12-2009

During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public research, with major impact on the fields of drug discovery, molecular medicine and personalized health care...

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-09-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where individual samples are screened against many thousands of target genes. According to its superior sensitivity and accuracy, qPCR is a well-established gold standard for precise profiling of gene expression levels or genotyping of known SNPs...

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New Products for Cell Culture Analysis from Roche Innovatis

31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More information about Roche Innovatis products will appear in future issues of Biochemica...

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Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR

04-06-2008

Retroviral reverse transcriptases commonly used for cDNA synthesis exhibit a higher error rate than other DNA polymerases used in nucleic acid analysis techniques. This lack of accuracy leads to a significant number of base exchanges or frameshifts which are further propagated in a subsequent PCR reaction. With the introduction of a new high-accuracy reverse transcriptase, Roche Applied Science now offers a new tool to increase the fidelity of cDNA synthesis. The Transcriptor High Fidelity cDNA Synthesis Kit not only reduces the amount of errors in RT-PCRs; it also allows the reverse transcription of full-length cDNAs with high yield....

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Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent

22-05-2008

The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid DNA, RNA, siRNA, and miRNA) into eukaryotic cells [1,2]. However, the optimal condition for incorporation depends on the type of transfection reagent and the cells being used, resulting in variable transfection efficiency and the extent of cell damage caused by transfection....

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Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System

19-09-2007

One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets....

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Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

12-09-2007

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de Génomique Fonctionnelle, Département d'Oncologie Moléculaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, Hôpital Carémeau, CHU Nîmes, France *Corresponding author...

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