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6 Current white paper about the topic fluorescence

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Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules

Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega


The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured in 96-well format...


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Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlokTMand FLUOstar OPTIMA

Real-time analysis of tumour cell invasion with a FLUOstar OPTIMA enclosed in an AtmosBag


The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok(TM) tumour cell invasion system. In this application note we show that the results are comparable to the traditional invasion assays. The introduced method is fast, saving time and labour. The FLUOstar OPTIMA is able to take multiple readings over time at a user defined temperature...


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New Standard in Electrophysiology and Deep Tissue Imaging

The Confocal Fixed Stage System Leica DM6000 CFS


The function of nerve and muscle cells relies on ionic currents flowing through ion channels. These ion channels play a major role in cell physiology. One way to investigate ion channels is to use patch clamping. This method allows investigation of ion channels in detail and recording of the electric activity of different types of cells, mainly excitable cells like neurons, muscle fibres or beta cells of the pancreas. The patch clamping technique was developed by Erwin Neher and Bert Sakmann in the 1970s and 80s to study individual ion channels in living cells. In 1991 they received the Nobel Prize for Physiology and Medicine for their work. Today the patch clamping technique is one of the most important methods in the field of electrophysiology...


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Interference and HAMA problems

Recognizing and avoiding of false results


Immunoassays are bioanalytical methods based on antibodies. These include ELISA, EIA, RIA, Western blotting, protein arrays, lateral flow assays (immuno-chromatography), immunohistochemistry or immuno-PCR. Antibodies detect the substances (analytes) in immunoassays. This detection is always highly specific and only the analytes are detected and bound. This is the theory! Practically any antibody not only binds its analyte. It also binds several other substances, but with very different affinities....


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Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent


The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid DNA, RNA, siRNA, and miRNA) into eukaryotic cells [1,2]. However, the optimal condition for incorporation depends on the type of transfection reagent and the cells being used, resulting in variable transfection efficiency and the extent of cell damage caused by transfection....


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Efficient Transfection of 293T Cell Line with Lentiviral Vectors Using FuGENE® HD Transfection Reagent


Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T cells with lentiviral vectors with high efficiency....


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