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14 Current white paper about the topic fluorescencerss
On the RealTime ready Configurator, we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL). The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing modified bases in ...
Smart Solutions for large Molecules
As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmace
Although congenital heart malformations are the most common birth defects in humans, the underlying pathomechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, NKX2-5) have been ...
The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...
When working with DNA and oligo samples great care needs to be taken at every stage to ensure that degradation does not occur, and the highly potent samples are not contaminated. Use of state of the art concentration systems can significantly speed up concentration rates, saving researchers time, ...
Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-fo
The experimental method of introducing foreign molecules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid ...
For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic ...
Samples by Reflection Microscopy
Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH ...
Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T ...