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62 Current white paper about the topic fluorescence

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RealTime ready RT-qPCR Assay Development and Qualification

01-06-2011

On the RealTime ready Configurator, we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL). The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing modified bases in ...

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RealTime ready qPCR Assay Design and Configuration Portal Content

01-06-2011

The gene and transcript annotational content of the RealTime ready Configurator offers a combined view of three of the main gene annotation authorities. The positional information of the lab-tested RealTime ready assays regarding transcript or exon coverage is presented in a graphical overview ...

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Agrisera Western Blot recommendations, troubleshooting and protocol

24-11-2010

Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number of ...

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Characterizing Biotherapeutics with HPLC

Smart Solutions for large Molecules

24-11-2010

As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmaceutical to approval at the regulatory agencies. ...

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High-Throughput Cardiac Gene Expression Analysis Using the Universal ProbeLibrary and the Novel LightCycler® 1536 Real-Time PCR System

25-01-2010

Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, NKX2-5) have been ...

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Major Histocompatibility Complex Genotyping with Massively Parallel Pyrosequencing

04-01-2010

Macaques provide essential preclinical models for infectious disease, vaccine, biodefense, and transplantation research. Unfortunately, they also have the most complex MHC genetics of any primate species described to date, and existing methods for MHC typing are simply inadequate. As shown here, ...

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Expression Analysis of ABC Transporters in Lung Cancer Cell Lines Using a RealTime ready qPCR Focus Panel

16-12-2009

The novel small molecule inhibitors are often substrates of ABC transporters which influence the pharmacokinetic parameters of the drug candidates and cause drug resistance of tumor cells. Therefore expression profiling of ABC transporter genes in cell lines used for drug screening is highly ...

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RealTime ready - Function Tested qPCR Assays Based on the Universal ProbeLibrary Technology

Gene expression profiling of the human genome on the LightCycler® 480 System has never been so easy.

14-12-2009

RealTime ready qPCR Assays redefine real-time qPCR because each assay is function-tested in PCR and qualified under strict criteria to assure superior performance. The RealTime ready Assays are based on the well-known Universal ProbeLibrary (UPL) technology and are available in a ready-to-use ...

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Altered Expression of Broad Range of Protease Genes in SH-SY5Y Neuroblastoma Cells after Bortezomib Exposure

14-12-2009

Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to neuroblastoma cells exposed to bortezomib, a ...

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