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26 Current white paper about the topic fluorescence

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GC-C-IRMS Determination of Hormones

eVol® Reduces Interference Caused by Solvents and Plastic Pipette Tips

26-09-2011

By amending methodology to utilize eVol® for sample preparation processes for GC-C-IRMS C isotope ratio analysis, the ISORA department has: improved sample preparation conditions and the GC-C-IRMS analysis results by removing the risk of ghost peaks, and increased their workflow, been able to ...

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Agrisera Western Blot recommendations, troubleshooting and protocol

24-11-2010

Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number of ...

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Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization

22-09-2010

High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar mass ...

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Functional Analysis of Side Effects of Transfection Reagents in the Context of Bax-induced Apoptosis

11-01-2010

FuGENE® HD Transfection Reagent is well-suited for studying the impact of apoptosis-inducing proteins such as bax. Transfection with FuGENE® HD Transfection Reagent produced only minimal cytotoxic side effects while maintaining a high transfection efficiency. In contrast, cytotoxicity, and ...

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Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase

25-11-2009

Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic ...

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ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader

15-09-2009

In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species (ROS). ROS are necessary intermediates in the human body, but they are also involved in the aging process and in the development of ...

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New Products for Cell Culture Analysis from Roche Innovatis

31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...

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Improvement of power generation efficiency of solar cells and evaluation by fluorescence spectroscopy

24-08-2009

The light in the high-radiance wavelength region of the solar spectrum is not utilized effectively by silicon crystal-based solar cell modules. "Fluorescent materials" convert the ultraviolet component in the sunlight into light in the longer wavelength region where this solar cell modules can ...

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Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus

Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation

27-04-2009

Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology ...

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Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer

25-08-2008

Nicotinamide Adenine Dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADP+) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) have been known to play vital roles in energy metabolism, antioxidation, and reductive ...

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