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8 Current white paper about the topic fluorescence

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Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase


Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic measurements were done on a FLUOstar OPTIMA and Km and Vmax values were calculated for each inhibitor using the MARS Data Analysis software...


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ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader


In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species (ROS). ROS are necessary intermediates in the human body, but they are also involved in the aging process and in the development of many degenerative diseases, including cancer, heart disease, Alzheimer's and Parkinson's. Herein we describe the determination of the antioxidant capacity of different fruit juices using the ORAC assay; a standardized method that uses Trolox®, a water-soluble analogue of vitamin E, as a standard substance...


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New Products for Cell Culture Analysis from Roche Innovatis


The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More information about Roche Innovatis products will appear in future issues of Biochemica...


Improvement of power generation efficiency of solar cells and evaluation by fluorescence spectroscopy


The light in the high-radiance wavelength region of the solar spectrum is not utilized effectively by silicon crystal-based solar cell modules. "Fluorescent materials" convert the ultraviolet component in the sunlight into light in the longer wavelength region where this solar cell modules can generate power. This article describes an example, how the efficiency of a fluorescent material can be evaluated, using a fluorescence spectrophotometer equipped with an integrating sphere, and a special program, which allows the determination of quantum yields of powders in a very comfortable way....


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Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus

Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation


Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology utilizes oxygen to start a cascade of chemical reactions resulting in a luminescence signal at 615 nm. Sensitivity and Z´values obtained in different plate formats are presented....


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Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System


The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation....


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Confocal Detection of NBT/BCIP In Situ Hybridization

Samples by Reflection Microscopy


Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH samples and confocal detection by reflection microscopy. WMISH protocols can also be combined with fluorescent counterstainings such as DAPI, antibodies, GFP lines, and fluorescent in situ probes. Such double and triple labeling combined with confocal microscopy allows expression profiling at a cellular resolution in cell types or embryological structures of interest. Whole-mount reflection CLSM will thus greatly facilitate large-scale, cellular resolution expression profiling in vertebrate and invertebrate model organisms....


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The Determination of Hazardous Substances According to RoHS and WEEE Directives


1st July 2006 is an important deadline for the electro- and electronics industries. This date bans hazardous substances like lead, cadmium, mercury, hexavalent chromium and polybrominated biphenyls and diphenyl ethers from electro- and electronic equipment. This restriction, even though in discussion since 1991, has become more and more important from year to year because of tons of electronic waste releasing toxic substances which are accumulated in the environment, the food chain and all human beings. In order to enforce the substances ban and the limitation or substitution of hazardous components, elemental analysis is obviously the most important control measure for monitoring limiting values. This requires precise analytical systems such as X-ray fluorescence, ICP- and atomic absorption spectrometers. These instruments are able to detect trace concentrations of hazardous compounds - for example cadmium, using an atomic absorption spectrometer in the flame atomization mode up to 0.1 mg/L, or using the di...


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