Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...

more

Smart Solutions for large Molecules

24-11-2010

Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...

more

23-06-2010

Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...

more

23-06-2010

alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells Cell proliferation on different substrates was followed over 17 days with the FLUOstar OPTIMA Fluorescent and colorimetric detection possible Introduction A variety of methods have ...

more

16-06-2010

Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...

more

A Cross-Platform Comparison

09-12-2009

Introduction During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public ...

more

25-11-2009

Pseudomonas elastase activity monitored using Abz-Nba internally quenched substrate Km and Vmax calculated using MARS Data Analysis Software Michaelis Menten kinetics determined for a library of 160 elastase inhibitors Introduction Pseudomonas elastase (pseudolysin, LasB) is a ...

more

15-09-2009

Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and 384-well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism ...

more

31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...

more

24-08-2009

The light in the high-radiance wavelength region of the solar spectrum is not utilized effectively by silicon crystal-based solar cell modules. "Fluorescent materials" convert the ultraviolet component in the sunlight into light in the longer wavelength region where this solar cell modules ...

more