Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...

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Smart Solutions for large Molecules

24-11-2010

Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...

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23-06-2010

Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...

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23-06-2010

alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells Cell proliferation on different substrates was followed over 17 days with the FLUOstar OPTIMA Fluorescent and colorimetric detection possible Introduction A variety of methods have ...

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16-06-2010

Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...

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15-09-2009

Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and 384-well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism ...

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07-09-2009

Introduction The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) ...

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31-08-2009

The Roche Innovats product line is now available from Roche Applied Sciences. Below is a short introduction to three Roche Innovatis product lines designed for the complete analysis of cell cultures, from the simple determination of cell concentration to complex cellular screening. More ...

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24-08-2009

The light in the high-radiance wavelength region of the solar spectrum is not utilized effectively by silicon crystal-based solar cell modules. "Fluorescent materials" convert the ultraviolet component in the sunlight into light in the longer wavelength region where this solar cell modules ...

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Real-time analysis of tumour cell invasion with a FLUOstar OPTIMA enclosed in an AtmosBag

24-06-2009

Introduction The mechanism of prostate metastasis to bone marrow is a complex multistage process that is only beginning to be understood. Initial steps include down regulation of molecular binding complexes and production of enzymes such as matrix metalloproteinases (1) leading to the loss of ...

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