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29 Current white paper about the topic fluorescence

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Process optimization made easy: design of experiments with multi-bioreactor system BIOSTAT® Qplus

The BIOSTAT® Qplus with up to 12 culture vessels represents the basis for a professional and time-saving process optimization.

22-10-2013

Design of experiments (DoE) is one of the most important techniques for systematic planning, execution and statistical evaluation of experiments. Although a DoE investigation is executable in one bioreactor, multi-bioreactor systems designed for parallel operation provide the optimal basis ...

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Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules

Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured in 96-well format

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alamarBlue® Assay for Assessment of Cell Proliferation using the FLUOstar OPTIMA

23-06-2010

The alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells. Active cells are able to convert resazurin into the resorufin. Resazurin is blue and non-fluorescent whereas resorufin is red and highly fluorescent; therefore a fluorescent and a ...

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Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega

23-06-2010

The POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in 96-well format. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid ...

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Application of the Amplifluor® SNPs Genotyping System using a FLUOstar OPTIMA microplate reader

16-06-2010

The detection of SNPs (single nucleotide polymorphisms) is an important tool for researchers in order to find mutations in DNA sequences. In this application note we describe a homogeneous fluorescence assay based on PCR. The Amplifluor® SNPs HT Genotyping System from Millipore was used to screen ...

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LightCycler® 480 and 1536 Real-Time PCR Systems - Powerful Solutions for Different Throughput Levels

A Cross-Platform Comparison

09-12-2009

During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public research, with major ...

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Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase

25-11-2009

Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic ...

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ORAC Assay Performed on the POLARstar Omega and PHERAstar FS microplate reader

15-09-2009

In all oxygen consuming cells, metabolism and oxidative stress generate several intermediates and byproducts that are collectively known as reactive oxygen species (ROS). ROS are necessary intermediates in the human body, but they are also involved in the aging process and in the development of ...

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High-Throughput Analysis Using the Novel LightCycler® 1536 Real-Time PCR System

07-09-2009

An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional genomics, where ...

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Adaptation of SmartAmp-2 Assays for Microsatellite Copy Number Polymorphism Detection on the LightCycler® 480 Instrument

07-09-2009

The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) protein in an ...

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