Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...

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23-06-2010

Membrane fluidity is measured in both liposomes and mitochondrial membranes via fluorescence polarization UV FP optics are used on the POLARstar Omega to measure DPH (1,6-diphenyl-hexa-1,3,5-triene) fluorescence The effects of dibucaine and propranolol on membrane fluidity are ...

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23-06-2010

alamarBlue® cell proliferation assay is based on a reducing environment that indicates metabolic active cells Cell proliferation on different substrates was followed over 17 days with the FLUOstar OPTIMA Fluorescent and colorimetric detection possible Introduction A variety of methods have ...

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16-06-2010

Bioscience Genotyping and Mapping Division, The Australian National University SNPs were easily detected with the Amplifluor® System from Millipore Detailed protocol and instrument settings are shown for this assay BMG LABTECH’s FLUOstar OPTIMA has proved to be an excellent reader for this ...

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A Cross-Platform Comparison

09-12-2009

Introduction During the past few years real-time PCR has been proven to be a key technology for gene expression analysis, for instance for gene expression profiling and array verification studies and for the genotyping of single nucleotide polymorphisms, both in industrial and public ...

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25-11-2009

Pseudomonas elastase activity monitored using Abz-Nba internally quenched substrate Km and Vmax calculated using MARS Data Analysis Software Michaelis Menten kinetics determined for a library of 160 elastase inhibitors Introduction Pseudomonas elastase (pseudolysin, LasB) is a ...

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15-09-2009

Antioxidants are able to neutralize Reactive Oxygen Species Antioxidant capacity of fruit juices determined with the ORAC assay in 96 and 384-well format MARS data analysis software with predefined templates for quick ORAC evaluation Introduction In all oxygen consuming cells, metabolism ...

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07-09-2009

Introduction The Smart Amplification process, version 2 (SMAP-2), also known as SmartAmp-2, is a rapid polymorphism detection system based on a unique asymmetric primer design, including a folding primer (FP) and a turn-back primer (TP) [1]. Combined with Thermus aquaticus MutS (Taq MutS) ...

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07-09-2009

Introduction An important trend in biological and biochemical analysis over the past 15 years has been miniaturization and parallelization of analytical procedures. Large-scale high-throughput analysis of gene expression or genetic polymorphisms is an essential element of modern functional ...

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24-08-2009

The light in the high-radiance wavelength region of the solar spectrum is not utilized effectively by silicon crystal-based solar cell modules. "Fluorescent materials" convert the ultraviolet component in the sunlight into light in the longer wavelength region where this solar cell modules ...

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