01-06-2011

Abstract The RealTime ready Configurator is a web-based configuration and ordering portal for function tested, custom RT-qPCR assays based on Universal ProbeLibrary (UPL) technology. It contains consolidated gene annotation information derived from three key public gene annotation resources ...

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01-06-2011

Abstract On the RealTime ready Configurator [1], we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL) [2]. The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing ...

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Smart Solutions for large Molecules

24-11-2010

Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...

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24-11-2010

Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number ...

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25-01-2010

Introduction Although congenital heart malformations are the most common birth defects in humans, the underlying patho­mechanisms remain widely unknown. Through linkage analyzes and candidate-gene approaches, several gene mutations causing congenital heart defects (e.g., CITED2, GATA4, ...

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04-01-2010

Pyrosequencing of complementary DNA-PCR ampli­cons was used to determine comprehensive major histocompatibility complex (MHC) class-I genotypes in primates that provide essential preclinical models for studies of infectious disease, vaccine development, and transplantation. By sequence-based ...

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21-12-2009

FAQ: When was gene silencing first described? HelpCorner: In 2006, Andrew Z. Fire and Craig C. Mello received the Nobel Prize in Physiology and Medicine for their discovery of “RNA interference - gene silencing by double-stranded RNA”. “To their surprise, they found that double-stranded RNA ...

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16-12-2009

Introduction The novel small molecule inhibitors are often substrates of ABC transporters [1] which influence the pharmacokinetic parameters of the drug candidates and cause drug resistance of tumor cells [2,3]. Therefore expression profiling of ABC transporter genes in cell lines used for ...

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14-12-2009

Introduction Neuroblastoma cell lines can be models for the responsiveness of neuroblastomas to chemotherapy-induced cell death as well as for the sensitivity of untransformed neuro-nal cells to toxic side-effects of chemotherapy. Both phenom-ena are relevant to neuroblastoma cells exposed ...

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Gene expression profiling of the human genome on the LightCycler® 480 System has never been so easy.

14-12-2009

Function tested qPCR assays for human targets are now only a few clicks away, with the new RealTime ready Configurator. This free online assay selection and shopping tool can be used to order single assays (for up to 300 20-µl reactions) or to configure customized panels on LightCycler® 480 ...

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