Thioflavin T aggregation assay to determine antibody stability using the FLUOstar Omega

03-11-2011

Stress can cause denaturation and aggregation of antibodies Aggregation propensity of antibody candidates was measured using a fluorescence based assay in 96-well format Shaking stress initiated by the FLUOstar Omega and the addition of Teflon beads enabled a successful antibody ...

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14-10-2011

#img11015# SummeryBiomarkers are currently changing the way we diagnose and treat diseases. Today pharmacodynamic biomarkers are widely used in the industry elaborating optimal dosing of new drugs and predictive biomarkers have an enormous potential for companion diagnostics ...

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Smart Solutions for large Molecules

24-11-2010

Development and production of biopharmaceuticals is a growing segment of pharmaceutical industry. Therapeutic proteins include blood factors, thrombolytic agents, growth factors, interferons, interleukins, therapeutic enzymes and last but not least monoclonal antibodies. More than twenty ...

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24-11-2010

Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number ...

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11-01-2010

In this study, the xCELLigence System was used to determine and characterize cell death after transfection of the bax gene into HeLa cells using three different commercially available transfection reagents. With the workflow presented here, it was possible not only to evaluate and compare the ...

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Using Liberase TL Research Grade

04-01-2010

In this study, the performance of Liberase TL Research Grade for the isolation of early progenitor cells from rat pancreatic tissue was analyzed. Tissue digestion with Liberase TL Research Grade resulted in high yields of isolated cells. Markers for the three germ layers (ecto-, endo- and ...

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Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation

27-04-2009

Introduction Immunoglobulins or antibodies are proteins that are involved in the immune response. They bind with high affinity at exogenous substances (antigens). Immunoglobulins (Ig) are classified according to the structure. The most present antibody in plasma is IgG. It is part of the ...

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Recognizing and avoiding of false results

16-06-2008

Immunoassays are bioanalytical methods based on antibodies. These include ELISA, EIA, RIA, Western blotting, protein arrays, lateral flow assays (immuno-chromatography), immunohistochemistry or immuno-PCR. Antibodies detect the substances (analytes) in immunoassays. This detection is always ...

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10-06-2008

General: Definitions and Techniques FAQ: How can I determine how fit my cells are? HelpCorner: Usually, one of two parameters is used to determine the health of the cells: cell viability or cell proliferation. Cell viability can be defined as the number of healthy cells in a sample. Cell ...

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Long-term stabilization of assay components

02-06-2008

Reliable long-term stabilization of immunoassay components is beneficial in many applications. Stabilization and storage of components are important for effective workflows not only for performing large numbers of ELISA but also for medium and small numbers of ELISA. This article deals with ...

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