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In vitro System Measures Cellular Responses to Oxygen Changes During Ischemia and Reperfusion

Microplate Reader Imitates Oxygen Removal And Recovery Of A Vascular Closure

Andrea Krumm

Example of ischemia-reperfusion atmospheric conditions in the CLARIOstar microplate reader with ACU

The lack of oxygen supply accompanies life-threatening diseases such as stroke, myocardial infarction or renal failure. Tissues and cells are temporarily not provided with O2 (ischemia) and have to develop survival-strategies. Apart from this, cells are exposed to a second threat upon re-oxygenation (reperfusion). Though being pivotal for survival, the sudden reperfusion of tissue induces oxidative stress and inflammatory responses.

Investigation of ischemia-reperfusion effects requires an experimental set-up that imitates rapid changes in O2 environment while recording its biological effects.

The ischemia-reperfusion model presented here uses a microplate reader with software-controlled O2 and CO2 regulation. Changes in oxygenation of HepG2 cells and Cor.4U® cardiomyocytes were reported by an intracellular oxygen-sensitive probe (MitoXpress®-Intra, Luxcel Biosciences).

Facts, background information, dossiers
  • Bmg Labtech
  • ischemia reperfusion
  • reactive oxygen species
  • Deoxygenation
  • oxygenation
  • oxygen
More about BMG Labtech
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