DNA isolation from anti-coagulated whole blood for subsequent molecular analysis by PCR methods is well-established. In a standard laboratory setting, the blood is shipped in a blood collection vial containing the anticoagulant after phlebotomy. However, it might not always be possible to have blood samples shipped immediately, and for longer storage cooling is required. Freezing anticoagulated blood samples might interfere with the efficiency of DNA isolation afterwards. Thus, the collection and storage of whole blood samples on special filter paper has become more and more popular since it provides a simple and easy method to ship and to store the samples. Evaluation of established DNA isolation kits and procedures is required in order to recommend a protocol for DNA isolation from dried blood spots (DBS). Here, we report the assessment of the High Pure PCR Template Preparation Kit for DNA Isolation from DBS more