Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

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New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

New Generation SEC Columns for Small-Scale Preparative Purification and Analysis of Proteins

The new generation size exclusion chromatography columns called “Increase” from GE Healthcare offer improved performance than their predecessors.  Comprising smaller, more rigid beads with a narrower particle size distribution and a higher selectivity than their predecessors, they offer: Reduced runtimes with maintained resolution for results faster Higher resolution for improved purity Higher lot to lot consistency The columns are tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over With the addition of Superdex™ 30 Increase in June 2017, you now have four choices of the new generation SEC resin for small-scale preparative purification and analysis (4 to 500 µl): For peptides and other small biomolecules: Superdex 30 Increase (Fractionation range Mr 100 to 7000) For recombinant tagged proteins: Superdex 75 Increase (Fractionation range Mr 3000 to 70 000) For MAbs and other antibodies: Superdex 200 Increase (Fractionation range Mr 10 000 to 600 000 For larger proteins and protein complexes: Superose™ 6 Increase (Fractionation range Mr 5000 to 5 000 000) Three of the resins (Superdex 75 Increase, Superdex 200 Increase and Supersoe 6 Increase) are available prepacked in high-performance columns of three different sizes:10 mm x 300 mm (24 ml column volume), 5 mm x 150 mm (3 ml column volume) and 3.2 mm x 300 mm (2.4 ml column volume). Superdex 30 Increase is available as 10 mm x 300 mm and 3.2 mm x 300 mm columns. All columns are made of glass to allow easier visual inspection of the packed bed. The columns have fittings for simple connection to ÄKTA™ chromatography systems or equivalent high-performance systems. Three different column dimensions are available: The 10 × 300 mm column is well suited for high resolution separations for both preparative and analytical purposes, with sample volumes from 25 µl to 500 µl For smaller sample volumes (4–50 µl) and high resolution preparations and analyses, the 3.2 × 300 mm column is the best choice The shorter 5 × 150 mm column is suitable for quick purity checks and rapid screening when the highest resolution is not needed Check what our customers are saying on the product website.

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

5+ products
4 brochures
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Chromatography explained

Affinity chromatography

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Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

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Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

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Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

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Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

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Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

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Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

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Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

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High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

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Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

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Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

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Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

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Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

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The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

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Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

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