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  • DKSH Performance Materials: Introduction of new program to source products

    Even under pressure to reduce costs, effective cooperation and hard work will always find a way to fulfill expectations. Most global players in the pharmaceutical industry are facing dramatic changes: blockbuster products launched in the 1990s will lose their patents over the next few years and face competition which will threaten their steady earnings more

  • Efficient Transfection of 293T Cell Line with Lentiviral Vectors Using FuGENE® HD Transfection Reagent

    Lentiviral vectors have become a powerful tool for gene transfer and genetic manipulation in research. Efficiency of the transfection reagent is most critical to getting a high-titer lentiviral recombinant virus. Here, we show that FuGENE® HD Transfection Reagent can be used to transfect 293T cells with lentiviral vectors with high efficiency. more

  • Transfecting Mouse Fibroblast NIH 3T3 Cells with Lipid Reagents - A Comparative Study

    Prior to the advent of transfection lipids, mouse fibroblast NIH 3T3 cells were essentially untransfectable and other methods such as retroviral gene delivery had to be employed to circumvent this problem. Although retroviruses can be used for transgene expression in 100% of cells, the expression is often low. Additionally, NIH 3T3 cells are traditionally used for cellular transformation studies, and creating oncogenic retroviruses is not ideal due to bio-safety concerns. Recently, lentivruses have been used for high gene expression in cells (up to 100%). However, this method causes even greater bio-safety concerns and requires updating culture facilities to bio-safety level 2+, resulting in a costly and time-consuming procedure. more

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