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  • To Probe or Not to Probe

    In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer prob­lems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrument we were able to solve the primer-dimer problem, increase PCR efficiency, and continue our investigations. more

  • Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent

    Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient transfection, molecules do not integrate into the host cell genome and are only expressed for approximately 24 hours - 96 hours post-transfection. more

  • FuGENE® HD Transfection Reagent Provides High Transfection Efficiency in the Murine NB2a Cell Line

    Cell transfection is a method of gene delivery that introduces foreign DNA into a cell. It is one of the most important approaches in modern genetic and proteomic research. With intriguing properties, transfection of primary cells is required for tissue engineering and gene therapy. However, one major problem is that many ­methods do not work for neural cell lines. Here we report that FuGENE® HD Transfection Reagent provides a high level of transfection efficiency and intracellular protein expression in murine neuroblastoma cells, the NB2a cell line. An optimization test using different transfection reagents, including FuGENE® HD Transfection Reagent, FuGENE® 6 Transfection Reagent, and another liposome-mediated reagent, was performed for green fluores­cent protein (GFP) expression in NB2a cells through transient transfection. more

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ELMI laboratory equipment.

COMPANY : NEWS : CONTACT :   ELMI Product catalogue 2009 Rotator/Mixer RM-2 Intelli-Mixer is a combination of variable speed rotator and advanced vortex in one machine. Shakers DOS, S-302 and DRS Series of orbital and rocking laboratory shakers. Thermo-shakers DTS and MT2 Series of high precission l more

Enzymes and reagents for genetic engineering and Molecular biologicals from Bangalore Genei, Bangalo

   Techware Equipment . PRODUCT Techware Equipment - full pdf ● Blotting Equipment Hybridisation oven Semi Dry blotter E-Blotter System Electro Transfer Systems UV Cross linker   ● Electrophoresis Equipment Electrophoresis Systems & Accessories Submarine Electrophoresis System Vertical Electrophores more

upchurch zubehör

  Chromatographiebedarf Laborpumpen (Peristaltikpumpen) LABORATORY PUMPS INTRODUCTION Fluid handling is a common need in most labs. With smaller dispense requirements and longer-to-continuous duty operations, smaller more accurate and robust pumping solutions are necessary. To meet these needs, we n more

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