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33 Newest Publications about the topic pcr systems
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Multiplex real-time PCR for the detection and quantification of DNA from four transgenic soy Mon89788, A5547-127, Roundup Ready, A2704-12 and lectin
04-23-2012 | René Köppel, Franziska van Velsen, Nora Felderer, Thomas Bucher, European Food Research and Technology, 2012
In the past, the transgenic soybean Roundup Ready was the predominantly cultivated transgenic soybean. With the announcement of Monsanto Corp. that this trait will be replaced by Mon89788 and the release of other company’s transgenic soy crops, this unique status of one trait has ended. ...
Event-specific qualitative and quantitative detection of transgenic rice Kefeng-8 by characterization of the transgene flanking sequence
01-06-2012 | Wei-xia Wang, Ting-heng Zhu, Feng-xiang Lai, Qiang Fu, European Food Research and Technology, 2012
The transgenic rice line Kefeng-8 harboring insect-resistant genes Cry1Ac and CpTI showed ideal field performances characterized by high resistance to rice stem borer (Scirpophaga incertulas) and leaf roller (Cnaphalocrocis medinalis). This GM rice line is likely to be approved in China in ...
Establishment and evaluation of event-specific qualitative and quantitative PCR method for genetically modified soybean DP-356043-5
08-26-2011 | Wen Tao Xu, Nan Zhang, Yun Bo Luo, Zhi Fang Zhai, Ying Shang, Xing Hua Yan, Juan Juan Zheng, Kun Lun Huang, European Food Research and Technology, 2011
With the increasing development of genetically modified organisms (GMOs), labeling regulations have been introduced, which require appropriate detection methods. The polymerase chain reaction (PCR) technique has been the mainstay for GMO detection, especially for event-specific qualitative ...
Quantitative detection of lactic acid bacteria in dried sourdoughs using real-time PCR
08-12-2011 | Melanie Wieschebrock, Marion Seitter, Christian Hertel, European Food Research and Technology, 2011
A real-time PCR system with 16S rRNA gene-targeted group-specific primers was developed to quantitatively detect lactic acid bacteria (LAB) of the genera Lactobacillus, Leuconostoc, Pediococcus, and Weissella in different types of commercially available dried sourdoughs. Despite a high degree ...
Measuring thermodynamic details of DNA hybridization using fluorescence
07-01-2011 | You, Yong; Tataurov, Andrey V.; Owczarzy, Richard, Biopolymers, 2011
Abstract Modern real‐time PCR systems make it easy to monitor fluorescence while temperature is varied for hundreds of samples in parallel, permitting high‐throughput studies. We employed such system to investigate melting transitions of ordered nucleic acid structures into disordered ...
Screening for vancomycin-resistant enterococci: an efficient and economical laboratory-developed test
06-09-2011 | European Journal of Clinical Microbiology & Infectious Diseases, 2011
Abstract A laboratory-developed test (Lab Assay), combining enrichment broth and real-time polymerase chain reaction (PCR) for vancomycin-resistant enterococci (VRE) screening, was developed and evaluated in this study. A total of 1,765 faecal or rectal swabs sent to the laboratory for ...
Construction of a Standard Reference Plasmid for Detecting GM Cottonseed Meal
04-11-2011 | Qingfeng Guan, Xiumin Wang, Da Teng, Yalin Yang, Fang Tian, Qingqiang Yin, Jianhua Wang, Applied Biochemistry and Biotechnology, 2011
A plasmid was constructed for quantification of genetically modified (GM) cottonseed meal in the gene-specific level. The Cry1Ab/c gene was connected with the Sad1 gene by fusion PCR. The fusion gene was cloned into the pMD®19-T Simple Vector. The plasmid DNA was then digested with a ...
The creatine kinase system and pleiotropic effects of creatine
03-29-2011 | Theo Wallimann, Malgorzata Tokarska-Schlattner, Uwe Schlattner, Amino Acids, 2011
The pleiotropic effects of creatine (Cr) are based mostly on the functions of the enzyme creatine kinase (CK) and its high-energy product phosphocreatine (PCr). Multidisciplinary studies have established molecular, cellular, organ and somatic functions of the CK/PCr system, in particular for ...
Comparison of a multiplexed MassARRAY system with real‐time allele‐specific PCR technology for genotyping of methicillin‐resistant Staphylococcus aureus
03-23-2011 | Syrmis, M. W.; Moser, R. J.; Whiley, D. M.; Vaska, V.; Coombs, G. W.; Nissen, M. D.; Sloots, T. P.; Nimmo, G. R., Clinical Microbiology and Infection, 2011
Clin Microbiol Infect Abstract The Sequenom MassARRAY iPLEX single‐nucleotide polymorphism (SNP) typing platform uses matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) coupled with single‐base extension PCR for high‐throughput multiplex SNP ...
Detection of Staphylococcus hyicus exfoliative toxin genes by dot blot hybridization and multiplex polymerase chain reaction
03-01-2011 | Onuma, Kenta; Uoya, Yusuke; Koide, Tetsuo; Shibata, Ayumi; Tanabe, Taishi; Sato, Hisaaki, Microbiology and Immunology, 2011
ABSTRACT We designed a novel DNA probe and novel PCR primer sets for detecting the genes coding for Staphylococcus hyicus (S. hyicus) exfoliative toxin (ET). In dot blot hybridization, the novel DNA probe hybridized with chromosomal DNA of ExhA‐, ExhB‐, ExhC‐, ExhD‐, and SHETA‐producing ...
