01-09-2012 | Jessica Wahlgren; Tanya De L. Karlson; Mikael Brisslert; Forugh Vaziri Sani; Esbjörn Telemo; Per Sunnerhagen; Hadi V ..., Nucleic Acids Research, 2012

Despite the promise of RNA interference (RNAi) and its potential, e.g. for use in cancer therapy, several technical obstacles must first be overcome. The major hurdle of RNAi-based therapeutics is to deliver nucleic acids across the cell’s plasma membrane. This study demonstrates that ...

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29-08-2012 | Jinliang Peng, Yonggang Zhao, Junhua Mai, Wei Guo, Yuhong Xu, The Journal of Gene Medicine, 2012

Abstract Background A major obstacle in applying gene therapy methods in experimental and clinical practice is the lack of safe and efficient gene delivery systems. Electroporation has been shown to an effective physical delivery method. A variety of factors have been shown to affect ...

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29-08-2012 | Simone Thomas, Sebastian Klobuch, Katrin Besold, Bodo Plachter, Jan Dörrie, Niels Schaft, Matthias Theobald, Wolfgan ..., European Journal of Immunology, 2012

Current protocols used to select CMV‐specific T cells for adoptive immunotherapy focus on virus‐specific memory T cells from seropositive donors. However, this strategy is not feasible in patients undergoing allogeneic hematopoietic stem‐transplantation (HSCT) from CMV‐seronegative donors. ...

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27-08-2012 | Damijan Miklavčič, Journal of Membrane Biology, 2012

Exposure of biological cells to a sufficiently strong external electric field results in increased permeability of cell membranes, referred to as “electroporation.” Since all types of cells (animal, plant and microorganism) can be effectively electroporated, electroporation is considered to ...

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24-08-2012 | Dashuai Mu et al., PLoS ONE, 2012

by Dashuai Mu, Liang Shi, Ang Ren, Mengjiao Li, Fengli Wu, Ailiang Jiang, Mingwen Zhao Ganoderma lucidum is one of the most important medicinal mushrooms; however, molecular genetics research on this species has been limited due to a lack of reliable reverse genetic tools. In this ...

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10-08-2012 | Damijan Miklavčič, Lluis M. Mir, P. Thomas Vernier, Journal of Membrane Biology, 2012

Introduction for the special issue on electroporation Content Type Journal Article Category Introduction Pages 1-2 DOI 10.1007/s00232-012-9473-z Authors Damijan Miklavčič, Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenija Lluis M. Mir, Université ...

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10-08-2012 | Peter Kramar, Lucie Delemotte, Alenka Maček Lebar, Malgorzata Kotulska, Mounir Tarek, Damijan Miklavčič, Journal of Membrane Biology, 2012

We present experimental and theoretical results of electroporation of small patches of planar lipid bilayers by means of linearly rising current. The experiments were conducted on ~120-μm-diameter patches of planar phospholipid bilayers. The steadily increasing voltage across the bilayer ...

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10-08-2012 | Mohammad Hjouj et al., PLoS ONE, 2012

by Mohammad Hjouj, David Last, David Guez, Dianne Daniels, Shirley Sharabi, Jacob Lavee, Boris Rubinsky, Yael Mardor Electroporation, is known to induce cell membrane permeabilization in the reversible (RE) mode and cell death in the irreversible (IRE) mode. Using an experimental ...

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01-08-2012 | Brown, C. Y., Eom, D. S., Amarnath, S., Agarwala, S., CSH Protocols, 2012

In ovo electroporation of chick embryos at ages ≥ E2 is simple to conduct and widely used to manipulate gene function. However, in ovo electroporation at early E1 stages has so far been unsuccessful because of unacceptable levels of tissue damage and embryonic lethality. Early E1 ...

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28-07-2012 | Marko Usaj, Masa Kanduser, Journal of Membrane Biology, 2012

The fusogenic state of the cell membrane can be induced by external electric field. When two fusogenic membranes are in close contact, cell fusion takes place. An appropriate hypotonic treatment of cells before the application of electric pulses significantly improves electrofusion ...

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