14-06-2013 | Jun Liu et al., PLoS ONE, 2013

by Jun Liu, Yan Luo, Hengtao Ge, Chengquan Han, Hui Zhang, Yongsheng Wang, Jianmin Su, Fusheng Quan, Mingqing Gao, Yong Zhang The present study was conducted to determine whether recombinant human β-defensin-3 (rHBD3) in the milk of transgenic goats has an anti-bacterial activity against ...

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11-06-2013 | Ola Weiland, Gustaf Ahl|[eacute]|n, Helmut Diepolder, Maria-Christina Jung, Sepideh Levander, Michael Fons, Iacob Ma ..., Molecular Therapy, 2013

Therapeutic DNA Vaccination Using In Vivo Electroporation Followed by Standard of Care Therapy in Patients With Genotype 1 Chronic Hepatitis C Molecular Therapy advance online publication, June 11 2013. doi:10.1038/mt.2013.119 Authors: Ola Weiland, Gustaf Ahlén, Helmut ...

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06-06-2013 | Deyou Xiao, Chenguo Yao, Huan Liu, Chengxiang Li, Jie Cheng, Fei Guo, Liling Tang, Bioelectromagnetics, 2013

Abstract The goal of this study was to assess the effect of nanosecond electric pulses on HepG2 human liver cancer cells. Electric pulses with a high strength of 10 kV/cm, duration of 500 ns and frequency of 1 Hz were applied to the cells. After delivery of electric pulses, apoptosis, ...

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01-06-2013 | Bates, M., Jones, S. A., Zhuang, X., CSH Protocols, 2013

Conventional fluorescence microscopy is limited by its spatial resolution, leaving many biological structures too small to be studied in detail. Stochastic optical reconstruction microscopy (STORM) is a method for superresolution fluorescence imaging based on the high accuracy localization ...

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24-05-2013 | Robert E. Neal et al., PLoS ONE, 2013

by Robert E. Neal, John H. Rossmeisl, John L. Robertson, Christopher B. Arena, Erica M. Davis, Ravi N. Singh, Jonathan Stallings, Rafael V. Davalos Irreversible electroporation (IRE) is a non-thermal focal ablation technique that uses a series of brief but intense electric pulses delivered ...

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01-05-2013 | David M. Feliciano; Shiliang Zhang; Jennifer L. Quon; Angélique Bordey, Human Molecular Genetics, 2013

Tuberous sclerosis complex (TSC) is a genetic disorder caused by mutations in TSC1 or TSC2 resulting in hyperactivity of the mammalian target of rapamycin and disabling brain lesions. These lesions contain misplaced neurons enriched in hypoxia-inducible factor 1a (HIF1a). However, the ...

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04-04-2013 | Yuji Atsuta, Ryosuke Tadokoro, Daisuke Saito, Yoshiko Takahashi, Development, Growth & Differentiation, 2013

Deciphering how the tubulogenesis is regulated is an essential but unsolved issue in developmental biology. Here, using Wolffian duct (WD) formation in chicken embryos, we have developed a novel method that enables gene manipulation during tubulogenesis in vivo. Exploiting that WD arises ...

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29-03-2013 | Tomohiko Nakata, Mikako Ito, Yoshiteru Azuma, Kenji Otsuka, Yoichiro Noguchi, Hirofumi Komaki, Akihisa Okumura, Kazu ..., Human Mutation, 2013

ABSTRACT Acethylcholinesterase (AChE) at the neuromuscular junction (NMJ) is mostly comprised of an asymmetric form in which three tetramers of catalytic AChE subunits are linked to a triple helical collagen Q (ColQ). Mutations in COLQ cause endplate AChE deficiency. We report three ...

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15-03-2013 | Yu-Hsiang Chang; John Connolly; Noriko Shimasaki; Kousaku Mimura; Koji Kono; Dario Campana, Cancer Research, 2013

Natural killer (NK) cells rely on surface receptors to distinguish healthy cells from cancer cells. We designed a receptor termed NKG2D-DAP10-CD3ζ that is composed of the NK cell activating molecule NKG2D plus 2 key signaling molecules, DAP10 and CD3ζ, and evaluated its capacity to promote ...

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07-03-2013 | Olivier Serralbo, Cyril A. Picard, Christophe Marcelle, genesis, 2013

ABSTRACT The use of shRNAmir to down‐regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, due to the limitations of electroporation‐mediated transgenesis, the down‐regulation of genes expressed at late stages ...

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