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202 Newest Publications about the topic dna extraction
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Specific and sensitive detection of Alcaligenes species from an agricultural environment
14-03-2013 | Miyo Nakano, Masumi Niwa, Norihiro Nishimura, Microbiology and Immunology, 2013
Abstract A quantitative real‐time PCR assay to specifically detect and quantify the genus Alcaligenes in samples from the agricultural environment, such as vegetables and farming soils, was developed. The minimum detection sensitivity was 106 fg of pure culture DNA, corresponding to DNA ...
Possible association between TGF‐β1 polymorphism and oral cancer
26-02-2013 | N. K. Carneiro, J. M. M. Oda, R. Losi Guembarovski, G. Ramos, B. V. Oliveira, I. J. Cavalli, E. M. S. F. Ribeiro, M. ..., International Journal of Immunogenetics, 2013
Summary Oral squamous cell carcinoma (OSCC) is a worldwide health problem because it is a great cause of cancer morbidity and mortality. The transforming growth factor‐β1 (TGF‐β1) is involved in the regulation of numerous immunomodulatory processes. Thus, the aim of this case–control ...
Efficient Methods for Targeted Mutagenesis in Zebrafish Using Zinc-Finger Nucleases: Data from Targeting of Nine Genes Using CompoZr or CoDA ZFNs
22-02-2013 | Raman Sood et al., PLoS ONE, 2013
by Raman Sood, Blake Carrington, Kevin Bishop, MaryPat Jones, Alberto Rissone, Fabio Candotti, Settara C. Chandrasekharappa, Paul Liu Recently, it has been shown that targeted mutagenesis using zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases ...
Alternative Recruitment Strategies Influence Saliva Sample Return Rates in Community‐Based Genetic Association Studies
14-02-2013 | Mahmood F. Bhutta, Lindsey Hobson, Jane Lambie, Elizabeth S. H. Scaman, Martin J. Burton, Henk Giele, Sarra E. Jamie ..., Annals of Human Genetics, 2013
Summary Collection of saliva for DNA extraction has created new opportunities to recruit participants from the community for genetic association studies. However, sample return rates are variable. No prior study has specifically addressed how study design impacts sample return. Using data ...
Optimisation of techniques for quantification of Botrytis cinerea in grape berries and receptacles by quantitative polymerase chain reaction
21-01-2013 | S. Saito, K.J. Dunne, K.J. Evans, K. Barry, L. Cadle‐Davidson, W.F. Wilcox, Australian Journal of Grape and Wine Research, 2013
Abstract Background and Aims Bunch rot symptoms can appear weeks after the infection of grape flowers by Botrytis cinerea. Quantitative polymerase chain reaction (qPCR) detects changes in the DNA mass of a target organism and is a potential tool for studying quiescent infections. The ...
Identification of ochratoxin A producing Aspergillus carbonarius and A. niger clade isolated from grapes using the loop‐mediated isothermal amplification (LAMP) reaction
18-01-2013 | Michelangelo Storari, Ramona Rudolf Rohr, Ilaria Pertot, Cesare Gessler, Giovanni A.L. Broggini, Journal of Applied Microbiology, 2013
Abstract Aims To develop two assays based on the loop‐mediated isothermal amplification (LAMP) of DNA for the quick and specific identification of Aspergillus carbonarius and ochratoxigenic strains of the Aspergillus niger clade isolated from grapes. Methods and Results Two sets ...
Specific and sensitive detection of Alcaligenes species from an agricultural environment
17-01-2013 | Miyo Nakano, Masumi Niwa, Norihiro Nishimura, Microbiology and Immunology, 2013
Abstract We developed a quantitative real‐time PCR assay to specificity detect and quantify the genus Alcaligenes in samples from the agricultural environment, such as vegetables and farming soils. The minimum detection sensitivity was 106 fg of pure culture DNA, corresponding to DNA ...
Rapid and sensitive diagnosis of Acanthamoeba keratitis by loop‐mediated isothermal amplification
12-01-2013 | Ge Zhao, Qing Yuan, Zicheng Song, Shiying Sun, Clinical Microbiology and Infection, 2013
Abstract A loop‐mediated isothermal amplification (LAMP) assay was developed for the detection of Acanthamoeba. The sensitivity of the LAMP assay was tested using different copies of positive DNA. The specificity of the assay was tested using DNA extracted from Acanthamoeba, Pseudomonas ...
Non‐invasive prenatal testing of fetal whole chromosome aneuploidy by massively parallel sequencing
09-01-2013 | Desheng Liang, Weigang Lv, Hua Wang, Liangpu Xu, Jing Liu, Haoxian Li, Liang Hu, Ying Peng, Lingqian Wu, Prenatal Diagnosis, 2013
ABSTRACT Objective To determine whether non‐invasive prenatal testing by maternal plasma DNA sequencing can uncover all fetal chromosome aneuploidies in one simple sequencing event. Methods Plasma samples from 435 pregnant women at high risk for Down syndrome were collected prior ...
UGT1A1 Gene Polymorphisms and the Toxicities of FOLFIRI in Chinese Han Patients with Gastrointestinal Cancer
01-01-2013 | Zhou, Chen-fei; Ma, Tao; Su, Yang; Ye, Zheng-bao; Ji, Jun; Yu, Ying-yan; Zhang, Jun; Liu, Bing-ya; Zhu, Zheng-gang, Anti-Cancer Agents in Medicinal Chemistry , 2013
Abstract: Background: Neutropenia and diarrhea are the common dose-limiting toxicities of irinotecan, and uridine diphosphate glucuronosyltransferases (UGTs) gene polymorphisms are considered to be one of the predictive markers of irinotecan related toxicities. This study aims to ...
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