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199 Newest Publications about the topic dna extraction
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Expanding the Diagnostic Use of PCR in Leptospirosis: Improved Method for DNA Extraction from Blood Cultures
11-08-2010 | Steen Villumsen et al., PLoS ONE, 2010
Background Leptospirosis is a neglected zoonosis of ubiquitous distribution. Symptoms are often non-specific and may range from flu-like symptoms to multi-organ failure. Diagnosis can only be made by specific diagnostic tests like serology and PCR. In non-endemic countries, leptospirosis is ...
A developed DNA extraction method for different soil samples
01-08-2010 | Hu, Yingchang; Liu, Zhiheng; Yan, Jianfang; Qi, Xiaohui; Li, Jing; Zhong, Shiqi; Yu, Jicheng; Liu, Qiu, Journal of Basic Microbiology, 2010
Four DNA extraction methods namely SDS‐hyperhaline method (I), modified SDS‐hyperhaline method (II), indirect method (III), alkaline lysis method (IV) were evaluated by comparing DNA yield, spectrophotometric quality, genomic integrity and PCR suitability in this paper. The results showed ...
Short rare MUC6 minisatellites‐5 alleles influence susceptibility to gastric carcinoma by regulating gene
01-08-2010 | Kwon, Jeong‐Ah; Lee, Sang‐Yeop; Ahn, Eun‐Kyung; Seol, So‐Young; Kim, Min Chan; Kim, Su J ..., Human Mutation, 2010
The human MUC6 gene, which is reported to be expressed in the stomach and gall bladder, is clustered on chromosome 11p15.5 with other secreted mucins. In this study, the genomic structure of MUC6 has been analyzed and five VNTR (minisatellites; MS1–MS5) were identified. These minisatellites ...
High sensitivity of both sequencing and real‐time PCR analysis of KRAS mutations in colorectal cancer tissue
01-08-2010 | Tol, Jolien; Dijkstra, Jeroen R.; Vink‐Börger, Marianne E.; Nagtegaal, Iris D.; Punt, Cornelis J.A.; Van ..., Journal of Cellular and Molecular Medicine, 2010
The KRAS mutation status predicts the outcome of treatment with epidermal growth factor receptor (EGFR) targeted agents, and therefore the testing for KRAS mutations has become an important diagnostic procedure. To optimize the quality of this test, we compared the results of the two most ...
Forage type and fish oil cause shifts in rumen bacterial diversity
01-08-2010 | Huws, Sharon A.; Lee, Michael R.F.; Muetzel, Stefan M.; Scott, Mark B.; Wallace, R. John; Scollan, Nigel D., FEMS Microbiology Ecology, 2010
Despite evidence supporting improved incorporation of beneficial polyunsaturated fatty acids (PUFA) into ruminant products, such as meat and milk, following red clover and fish oil (FO) inclusion in the ruminant diet, little is known regarding the concomitant bacterial diversity. We evaluated ...
Binding of lac repressor-GFP fusion protein to lac operator sites inserted in the tobacco chloroplast genome examined by chromatin immunoprecipitation
01-08-2010 | Newell, Christine A.; Gray, John C., Nucleic Acids Research, 2010
Chromatin immunoprecipitation (ChIP) has been used to detect binding of DNA-binding proteins to sites in nuclear and mitochondrial genomes. Here, we describe a method for detecting protein-binding sites on chloroplast DNA, using modifications to the nuclear ChIP procedures. The method was ...
Bacterial community analysis of activated sludge: an evaluation of four commonly used DNA extraction methods
23-07-2010 | Louise Vanysacker, Steven A. J. Declerck, Bart Hellemans, Luc De Meester, Ivo Vankelecom, Priscilla Declerck, Applied Microbiology and Biotechnology, 2010
The effectiveness of three commercially available direct DNA isolation kits (Mobio, Fast, Qiagen) and one published direct DNA extraction protocol (Bead) for extracting bacterial DNA from different types of activated sludge was investigated and mutually compared. The DNA quantity and purity ...
Rapid molecular methods for enumeration and taxonomical identification of acetic acid bacteria responsible for submerged vinegar production
23-07-2010 | Rocío Fernández-Pérez, Carmen Torres, Susana Sanz, Fernanda Ruiz-Larrea, European Food Research and Technology, 2010
The aim of the present study was to search for a rapid and reliable method to enumerate viable acetic acid bacteria (AAB) and to identify to genera and species level AAB isolates from vinegars in full acetic fermentation elaborated by the submerged method from cider, wine and spirit ethanol ...
MRSA screening: can one swab be used for both culture and rapid testing?An evaluation of chromogenic culture and subsequent Hain GenoQuick PCR amplification/detection
01-07-2010 | Sherlock, O.; Dolan, A.; Humphreys, H., Clinical Microbiology and Infection, 2010
Clin Microbiol Infect The use of a single swab for both MRSA culture and for rapid testing by PCR was evaluated, using the Hain GenoQuick (GQM) methicillin resistant Staphylococcus aureus (MRSA) assay for the rapid detection of MRSA, as a single swab would be the preferred option for routine ...
Development of loop‐mediated isothermal amplification and PCR assays for rapid and simple detection of Campylobacter fetus subsp. venerealis
01-07-2010 | Yamazaki, Wataru; Taguchi, Masumi; Misawa, Naoaki, Microbiology and Immunology, 2010
Campylobacter fetus is divided into CFV and CFF. Because CFV causes bovine genital campylobacteriosis, differentiation of the two subspecies is essential to the implementation of efficient CFV control and eradication programs. We have developed LAMP and duplex PCR assays for rapid and simple ...
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