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18,479 Newest Publications
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Understanding the Biological Context of NS5A–Host Interactions in HCV Infection: A Network-Based Approach
17-05-2013 | Lokesh P. Tripathi; Hiroto Kambara; Yi-An Chen; Yorihiro Nishimura; Kohji Moriishi; Toru Okamoto; Eiji Morita; Takay ..., Journal of Proteome Research, 2013
Hepatitis C virus (HCV) is a major cause of chronic liver disease. HCV NS5A protein plays an important role in HCV infection through its interactions with other HCV proteins and host factors. In an attempt to further our understanding of the biological context of protein interactions between ...
Thiol-ene Enabled Detection of Thiophosphorylated Kinase Substrates
17-05-2013 | Kathleen C. A. Garber; Erin E. Carlson, ACS Chemical Biology, 2013
Protein phosphorylation is a ubiquitous posttranslational modification that regulates cell signaling in both prokaryotes and eukaryotes. Although the study of phosphorylation has made great progress, several major hurdles remain, including the difficulty of the assignment of endogenous ...
Role for Chlamydial Inclusion Membrane Proteins in Inclusion Membrane Structure and Biogenesis
17-05-2013 | Jeffrey Mital et al., PLoS ONE, 2013
by Jeffrey Mital, Natalie J. Miller, David W. Dorward, Cheryl A. Dooley, Ted Hackstadt The chlamydial inclusion membrane is extensively modified by the insertion of type III secreted effector proteins. These inclusion membrane proteins (Incs) are exposed to the cytosol and share a common ...
Primary Cilia: The Chemical Antenna Regulating Human Adipose-Derived Stem Cell Osteogenesis
17-05-2013 | Josephine C. Bodle et al., PLoS ONE, 2013
by Josephine C. Bodle, Candace D. Rubenstein, Michelle E. Phillips, Susan H. Bernacki, Jie Qi, Albert J. Banes, Elizabeth G. Loboa Adipose-derived stem cells (ASC) are multipotent stem cells that show great potential as a cell source for osteogenic tissue replacements and it is critical to ...
Reduction of fat content during frying using dried egg white and fiber solutions
16-05-2013 | Robert G. Brannan, Andrew S. Myers, Christopher S. Herrick, European Journal of Lipid Science and Technology, 2013
Summary Producers of deep‐fried foods seek new methods to lower the fat content of these foods. The effectiveness of edible films of proteins and carbohydrates and the addition of prefrying dips of egg white lower fat absorption. Common additions of chemicals to films called plasticizers ...
Acquisition of thermotolerant yeast Saccharomyces cerevisiae by breeding via stepwise adaptation
16-05-2013 | Atsushi Satomura, Yoshiaki Katsuyama, Natsuko Miura, Kouichi Kuroda, Ayako Tomio, Takeshi Bamba, Eiichiro Fukusaki, ..., Biotechnology Progress, 2013
Abstract A thermotolerant Saccharomyces cerevisiae yeast strain, YK60‐1, was bred from a parental strain, MT8‐1, via stepwise adaptation. YK60‐1 grew at 40oC, a temperature at which MT8‐1 could not grow at all. YK60‐1 exhibited faster growth than MT8‐1 at 30oC. To investigate the ...
Both human ferredoxins equally efficiently rescue ferredoxin deficiency in Trypanosoma brucei
16-05-2013 | Piya Changmai, Eva Horáková, Shaojun Long, Eva Černotíková‐Stříbrná, Lindsay M. McDonald, Esteban J. Bontempi, Juliu ..., Molecular Microbiology, 2013
Summary Ferredoxins are highly conserved proteins that function universally as electron transporters. They not only require Fe‐S clusters for their own activity, but are also involved in Fe‐S formation itself. We identified two homologues of ferredoxin in the genome of the parasitic ...
Superoxide poisons mononuclear iron enzymes by causing mismetallation
16-05-2013 | Mianzhi Gu, James A. Imlay, Molecular Microbiology, 2013
Summary Superoxide (O2‐) is a primary agent of intracellular oxidative stress. Genetic studies in many organisms have confirmed that excess O2‐ disrupts metabolism, but to date only a small family of [4Fe‐4S] dehydratases have been identified as direct targets. This investigation reveals ...
A Novel RNA Polymerase‐Binding Protein Controlling Genes Involved in Spore Germination in Bacillus subtilis
16-05-2013 | Bjorn A. Traag, Arturo Ramirez‐Peralta, Anna F. Wang Erickson, Peter Setlow, Richard Losick, Molecular Microbiology, 2013
Summary A growing class of proteins regulates transcription through interaction with DNA‐dependent RNA polymerase. Here we report that a recently identified, highly conserved sporulation gene ylyA encodes a novel RNA polymerase‐binding protein that influences the expression of genes under ...
Ultradepletion of Human Plasma using Chicken Antibodies: A Proof of Concept Study
16-05-2013 | Sock-Hwee Tan; Abidali Mohamedali; Amit Kapur; Mark S. Baker, Journal of Proteome Research, 2013
Human plasma arguably represents the most comprehensive version of the human proteome. Despite its immense theoretical discovery potential, plasma has many high and medium abundance proteins that mask low abundance protein disease biomarkers of relevance, making the discovery of novel ...
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