01-01-2011 | Pierce, Michael; Kahn, Jennifer Nielsen; Chiou, Jiachi; Tumer, Nilgun E., RNA, 2011

Ricin produced by the castor bean plant and Shiga toxins produced by pathogenic Escherichia coli (STEC) and Shigella dysenteriae are type II ribosome inactivating proteins (RIPs), containing an enzymatically active A subunit that inhibits protein synthesis by removing an adenine from the ...

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01-01-2011 | Wyler, Emanuel; Zimmermann, Mirjam; Widmann, Barbara; Gstaiger, Matthias; Pfannstiel, Jens; Kutay, Ulrike; Zemp, Ivo, RNA, 2011

Tandem affinity purification (TAP) is an efficient method for the purification and characterization of large macromolecular complexes. To elucidate the role of specific components of such complexes, it is important to address the question of how loss of a specific factor affects complex ...

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01-01-2011 | Wunnicke, Dorith; Strohbach, Denise; Weigand, Julia E.; Appel, Bettina; Feresin, Emiliano; Suess, Beatrix; Müller, S ..., RNA, 2011

RNA aptamers are in vitro–selected binding domains that recognize their respective ligand with high affinity and specificity. They are characterized by complex three-dimensional conformations providing preformed binding pockets that undergo conformational changes upon ligand binding. Small ...

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01-01-2011 | Smalheiser, Neil R.; Lugli, Giovanni; Thimmapuram, Jyothi; Cook, Edwin H.; Larson, John, RNA, 2011

We previously proposed that endogenous siRNAs may regulate synaptic plasticity and long-term gene expression in the mammalian brain. Here, a hippocampal-dependent task was employed in which adult mice were trained to execute a nose-poke in a port containing one of two simultaneously present ...

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01-01-2011 | Schellenberg, Matthew J.; Dul, Erin L.; MacMillan, Andrew M., RNA, 2011

Human p14 (SF3b14), a component of the spliceosomal U2 snRNP, interacts directly with the pre-mRNA branch adenosine within the context of the bulged duplex formed between the pre-mRNA branch region and U2 snRNA. This association occurs early in spliceosome assembly and persists within the ...

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01-01-2011 | Tseng, Chi-Kang; Liu, Hsueh-Lien; Cheng, Soo-Chen, RNA, 2011

The assembly of the spliceosome involves dynamic rearrangements of interactions between snRNAs, protein components, and the pre-mRNA substrate. DExD/H-box ATPases are required to mediate structural changes of the spliceosome, utilizing the energy of ATP hydrolysis. Two DExD/H-box ATPases are ...

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01-01-2011 | Powrie, Erin A.; Zenklusen, Daniel; Singer, Robert H., RNA, 2011

The biogenesis of a localization-competent mRNP begins in the nucleus. It is thought that the coordinated action of nuclear and cytoplasmic components of the localization machinery is required for the efficient export and subsequent subcellular localization of these mRNAs in the cytoplasm. ...

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01-01-2011 | Nilsson, Daniel; Sunnerhagen, Per, RNA, 2011

Severe stress causes plant and animal cells to form large cytoplasmic granules containing RNA and proteins. Here, we demonstrate the existence of stress-induced cytoplasmic RNA granules in Schizosaccharomyces pombe. Homologs to several known protein components of mammalian processing bodies ...

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01-01-2011 | McCann, Michael D.; Lim, Geoffery F.S.; Manni, Michelle L.; Estes, Julie; Klapec, Kelly A.; Frattini, Gregory D.; Kn ..., RNA, 2011

Thirty-one RNA duplexes containing single-nucleotide bulge loops were optically melted in 1 M NaCl, and the thermodynamic parameters H°, S°, G°37, and TM for each sequence were determined. The bulge loops were of the group II variety, where the bulged nucleotide is identical to one of its ...

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01-01-2011 | Hasenöhrl, David; Konrat, Robert; Bläsi, Udo, RNA, 2011

In both Bacteria and Eukaryotes, degradation is known to start at the 5' and at the 3' extremities of mRNAs. Until the recent discovery of 5'-to-3' exoribonucleases in hyperthermophilic Euryarchaeota, the exosome was assumed to be the key enzyme in mRNA degradation in Archaea. By means of ...

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