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483 Newest Publications in plant cell, tissue and organ culture

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Primary and secondary somatic embryogenesis in Chrysanthemum cv. Euro

22-10-2012 | Aung Htay Naing, Chang Kil Kim, Baek Ji Yun, Jo Yu Jin, Ki Byung Lim, Plant Cell, Tissue and Organ Culture, 2012

Somatic embryogenesis was induced from in vivo grown leaf explants of Chrysanthemum cv. Euro incubated on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 2.0 mg/L Kinetin, yielding the highest mean number of embryos (42 ± 5.97) per explant after 5 ...

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CLAVATA1-LIKE, a leucine-rich-repeat protein receptor kinase gene differentially expressed during adventitious caulogenesis in Pinus pinaster and Pinus pinea

20-10-2012 | José M. Alvarez, Millán Cortizo, Natalia Bueno, Ana Rodríguez, Ricardo J. Ordás, Plant Cell, Tissue and Organ Culture, 2012

Molecular cloning and characterization of a CLAVATA1-LIKE gene in Pinus pinaster and Pinus pinea is reported. CLAVATA1 is a well-characterized gene in Arabidopsis integral to the balance between primordial differentiation and meristem proliferation. Currently, it is not known if the ...

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Expression of a cholera toxin B subunit and consensus dengue virus envelope protein domain III fusion gene in transgenic rice callus

20-10-2012 | Mi-Young Kim, Nguyen-Duc Chung, Moon-Sik Yang, Tae-Geum Kim, Plant Cell, Tissue and Organ Culture, 2012

Dengue (DEN) is one of the most important emerging mosquito-borne viral human diseases. Therefore, an effective dengue vaccine with immune responses against all four dengue virus serotypes is highly needed. A fusion gene encoding a synthetic consensus envelope protein domain III (scEDIII) of ...

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A reliable and efficient protocol for inducing hairy roots in Papaver bracteatum

19-10-2012 | Ali Sharafi, Haleh Hashemi Sohi, Amir Mousavi, Pejman Azadi, Khadijeh Razavi, Valentine Otang Ntui, Plant Cell, Tissue and Organ Culture, 2012

An efficient and reliable transformation system for a very important medicinal plant Papaver bracteatum was developed through optimization of several factors that affect the rate of effective A. rhizogenes-mediated transformation and growth rate of hairy root. Five bacterial strains, A4, ...

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Cellular aggregation is a key parameter associated with long term variability in paclitaxel accumulation in Taxus suspension cultures

08-10-2012 | Rohan A. Patil, Martin E. Kolewe, Susan C. Roberts, Plant Cell, Tissue and Organ Culture, 2012

Plant cell cultures provide a renewable source for synthesis and supply of commercially valuable plant-derived products, particularly for secondary metabolites. However, instability in product yields over multiple passages has hampered the efficient and sustainable use of this technology. ...

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Sonication-assisted efficient Agrobacterium-mediated genetic transformation of the multipurpose woody desert shrub Leptadenia pyrotechnica

06-10-2012 | Indrajit Dutta, Martin Kottackal, Ellen Tumimbang, Hiromi Tajima, Abdelouahhab Zaid, Eduardo Blumwald, Plant Cell, Tissue and Organ Culture, 2012

The desert shrub Leptadenia pyrotechnica (Forssk.) Decne (Asclepiadaceae) is an important multipurpose woody species of tropical and sub-tropical arid regions. The shrub’s excellent pharmacological properties, importance in desert afforestation and role in sand dune fixation has been ...

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Microspore embryogenesis and production of haploid and doubled haploid plants in carrot (Daucus carota L.)

17-09-2012 | Jin-Rong Li, Fei-Yun Zhuang, Cheng-Gang Ou, Hong Hu, Zhi-Wei Zhao, Ji-Hua Mao, Plant Cell, Tissue and Organ Culture, 2012

Protocols were developed for the generation of haploid and doubled haploid plants from isolated microspores of carrot (Daucus carota L.). Forty-seven carrot accessions, including six inbred lines, 11 cultivars, 20 F1s, two BC1F1s, four F2s, one F3, and three F4s, were screened to evaluate the ...

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Influence of fungal elicitation with Nomuraea rileyi (Farlow) Samson in the metabolism of acclimatized plants of Hypericumpolyanthemum Klotzsech ex Reichardt (Guttiferae)

16-09-2012 | Gabriela Meirelles, Amanda Valle Pinhatti, Daniel Sosa-Gomez, Luis Mauro Gonçalves Rosa, Sandra B. Rech, Gilsane Lin ..., Plant Cell, Tissue and Organ Culture, 2012

Changes in biomass and bioactive metabolites after treatment with the fungus Nomuraea rileyi (Farlow) Samson added as dried culture (DC) or as dried autoclaved cell powder (DACP) for short and long periods of time have been investigated in Hypericum polyanthemum plants grown under controlled ...

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Engineering production of antihypertensive peptides in plants

15-09-2012 | Sergio Rosales-Mendoza, Luz María Teresita Paz-Maldonado, Dania O. Govea-Alonso, Schuyler S. Korban, Plant Cell, Tissue and Organ Culture, 2012

To date, a number of antihypertensive peptides (AHPs) have been identified. Most of these are derived from proteins present in common edible consumables, including milk, egg, and plant foods. Consumption of these foods serves as means of AHP delivery and thus contributing favorable health ...

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Regulation of LaMYB33 by miR159 during maintenance of embryogenic potential and somatic embryo maturation in Larix kaempferi (Lamb.) Carr.

15-09-2012 | Wan-Feng Li, Shou-Gong Zhang, Su-Ying Han, Tao Wu, Jun-Hong Zhang, Li-Wang Qi, Plant Cell, Tissue and Organ Culture, 2012

During the process of subculture of embryogenic cultures, sometimes they may become non-embryogenic, which is not desirable. However, this offers an opportunity to explore the mechanisms underlying cell fate determination and the maintenance of embryogenic potential of explants during the ...

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