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483 Newest Publications in plant cell, tissue and organ culture

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Flow cytometric analysis and molecular characterization of Agrobacterium tumefaciens-mediated transformants of Medicago truncatula

27-11-2012 | S. Ochatt, L. Jacas, E. M. Patat-Ochatt, S. Djenanne, Plant Cell, Tissue and Organ Culture, 2012

Leaf explants from leaflets collected from either in vivo grown or in vitro grown seedlings of Medicago truncatula genotype R108-1 were co-cultivated with bacterial cells of Agrobacterium tumefaciens strains EHA105 or C58pMP90. Each of these strains was carrying the pCambia 1390 plasmid ...

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The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis

27-11-2012 | Xianjun Peng, Lexin Zhang, Lixing Zhang, Zhujiang Liu, Liqin Cheng, Ying Yang, Shihua Shen, Shuangyan Chen, Gongshe Liu, Plant Cell, Tissue and Organ Culture, 2012

S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the ...

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Agrobacterium-mediated transformation of alfalfa (Medicago sativa) using a synthetic cry3a gene to enhance resistance against alfalfa weevil

27-11-2012 | Masoud Tohidfar, Naser Zare, Gholamreza Salehi Jouzani, Seide Maryam Eftekhari, Plant Cell, Tissue and Organ Culture, 2012

To introduce genetic resistance against alfalfa weevil (Hypera postica), leaves and petiole explants of three commercial alfalfa genotypes, including Km-27, Kk-14 and Syn-18 were transformed with Agrobacterium tumefaciens strains GV101, LBA4404 and AGL01. All the Agrobacterium strains used ...

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Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii

26-11-2012 | Nohemí Campos-Quevedo, Sergio Rosales-Mendoza, Luz María Teresita Paz-Maldonado, Luis Martínez-Salgado, Juan Carlos ..., Plant Cell, Tissue and Organ Culture, 2012

Bioactive peptides are considered high value-added ingredients in functional foods, and the main sources of these are milk, egg, plants, among others. A major limitation in their commercial use is the cost of production. This study deals with the design and production of a chimeric protein in ...

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GsVAMP72, a novel Glycine soja R-SNARE protein, is involved in regulating plant salt tolerance and ABA sensitivity

19-11-2012 | Xiaoli Sun, Wei Ji, Xiaodong Ding, Xi Bai, Hua Cai, Shanshan Yang, Xue Qian, Mingzhe Sun, Yanming Zhu, Plant Cell, Tissue and Organ Culture, 2012

Abiotic stress, especially high salinity, is a major threat to agricultural production. It has been well established that SNARE proteins sustain directed vesicle traffic to underpin plant growth and development, yet little is known about the role of SNARE protein in the capacity to withstand ...

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Production of an antigenic C4(V3)6 multiepitopic HIV protein in bacterial and plant systems

19-11-2012 | Dania O. Govea-Alonso, Erik E. Gómez-Cardona, Néstor Rubio-Infante, Ana Lilia García-Hernández, Javier T. Varona-San ..., Plant Cell, Tissue and Organ Culture, 2012

A C4(V3)6 multiepitopic protein was designed in an effort to pursue broad immunization against the human immunodeficiency virus (HIV). This C4(V3)6 chimeric protein is based on sequences of gp120, including epitopes from the fourth conserved domain (C4) and six tandem repeats of the third ...

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Enhanced production of the alkaloid nicotine in hairy root cultures of Nicotiana tabacum L.

19-11-2012 | Bo Zhao, Foster A. Agblevor, Ritesh K. C., John G. Jelesko, Plant Cell, Tissue and Organ Culture, 2012

The utility of hairy root cultures to produce valuable phytochemicals could be improved by repartitioning more of the desired phytochemical into the spent culture media, thereby simplifying the bioprocess engineering associated with the purification of the desired phytochemical. The majority ...

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Phosphomannose-isomerase as a selectable marker for transgenic plum (Prunus domestica L.)

19-11-2012 | Hong Wang, César Petri, Lorenzo Burgos, Nuria Alburquerque, Plant Cell, Tissue and Organ Culture, 2012

A mannose selection system was adapted for Agrobacterium-mediated transformation of plum (Prunus domestica L.) hypocotyl explants and the recovery of transgenic plants. Adventitious regeneration from non-transformed hypocotyl sections was inhibited when 3 mg/l mannose, combined with 10 mg/l ...

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Molecular characterization of a stress-response bZIP transcription factor in banana

14-11-2012 | Sen He, Wei Shan, Jian-fei Kuang, Hui Xie, Yun-yi Xiao, Wang-jin Lu, Jian-ye Chen, Plant Cell, Tissue and Organ Culture, 2012

Increasing evidence has demonstrated that basic leucine zipper (bZIP) transcription factors (TFs) are functional and involved in plant stress response, but few bZIP TFs have thus far been investigated in economically important fruit crops, such as bananas. In our present work, a novel 43.4 ...

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Expression of a recombinant Human papillomavirus 16 E6GT oncoprotein fused to N- and C-termini of Potato virus X coat protein in Nicotiana benthamiana

11-11-2012 | Noemi Cerovska, Tomas Moravec, Hana Hoffmeisterova, Helena Plchova, Helena Synkova, Ingrid Polakova, Martina Duskova ..., Plant Cell, Tissue and Organ Culture, 2012

Infection with Human papillomaviruses (HPVs) is linked to cervical cancer, which is one of the most common cancers found among women worldwide. Despite preventive immunization, a therapeutic vaccine, targeting infected individuals, is required. Vaccine strategies for treatment of HPV—induced ...

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