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284 Newest Publications in plant biotechnology journal

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Marker‐free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii

07-05-2013 | Hsu‐Ching Chen, Anastasios Melis, Plant Biotechnology Journal, 2013

Summary The work applied a transgene expression method based on the replacement of an inactive rbcL gene as the selection marker in Chlamydomonas reinhardtii chloroplasts. The native rbcL gene in strain CC2653 has a point mutation that causes early translation termination, thus resulting ...

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A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus‐like particles

06-05-2013 | Eva C. Thuenemann, Ann E. Meyers, Jeanette Verwey, Edward P. Rybicki, George P. Lomonossoff, Plant Biotechnology Journal, 2013

Summary Plant expression systems based on nonreplicating virus‐based vectors can be used for the simultaneous expression of multiple genes within the same cell. They therefore have great potential for the production of heteromultimeric protein complexes. This work describes the efficient ...

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Sequence‐based SNP genotyping in durum wheat

03-05-2013 | Remco M.P. Poecke, Marco Maccaferri, Jifeng Tang, Hoa T. Truong, Antoine Janssen, Nathalie J. Orsouw, Silvio Salvi, ..., Plant Biotechnology Journal, 2013

Summary Marker development for marker‐assisted selection in plant breeding is increasingly based on next‐generation sequencing (NGS). However, marker development in crops with highly repetitive, complex genomes is still challenging. Here we applied sequence‐based genotyping (SBG), which ...

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Accelerating adoption of genetically modified crops in Africa through a trade liability regime

22-04-2013 | Stuart J. Smyth, William A. Kerr, Peter W. B. Phillips, Plant Biotechnology Journal, 2013

Summary Given the apparently unbridgeable divide that has developed between the 25 odd countries that grow and trade GM crops and the evolving EU regulatory hurdles, it may be time to consider alternative strategies for realizing a global market for agricultural products. Africa is one ...

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Induction of toxin‐specific neutralizing immunity by molecularly uniform rice‐based oral cholera toxin B subunit vaccine without plant‐associated sugar modification

22-04-2013 | Yoshikazu Yuki, Mio Mejima, Shiho Kurokawa, Tomoko Hiroiwa, Yuko Takahashi, Daisuke Tokuhara, Tomonori Nochi, Yuko K ..., Plant Biotechnology Journal, 2013

Summary Plants have been used as expression systems for a number of vaccines. However, the expression of vaccines in plants sometimes results in unexpected modification of the vaccines by N‐terminal blocking and sugar‐chain attachment. Although MucoRice‐CTB was thought to be the first ...

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Early anther ablation triggers parthenocarpic fruit development in tomato

15-04-2013 | Mónica Medina, Edelín Roque, Benito Pineda, Luis Cañas, Manuel Rodriguez‐Concepción, José Pío Beltrán, Concepción Gó ..., Plant Biotechnology Journal, 2013

Summary Fruit set and fruit development in tomato is largely affected by changes in environmental conditions, therefore autonomous fruit set independent of fertilization is a highly desirable trait in tomato. Here, we report the production and characterization of male‐sterile transgenic ...

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Expression of an Arabidopsis molybdenum cofactor sulphurase gene in soybean enhances drought tolerance and increases yield under field conditions

13-04-2013 | Yajun Li, Jiachang Zhang, Juan Zhang, Ling Hao, Jinping Hua, Liusheng Duan, Mingcai Zhang, Zhaohu Li, Plant Biotechnology Journal, 2013

Summary LOS5/ABA3 gene encoding molybdenum cofactor sulphurase is involved in aldehyde oxidase (AO) activity in Arabidopsis, which indirectly regulates ABA biosynthesis and increased stress tolerance. Here, we used a constitutive super promoter to drive LOS5/ABA3 overexpression in soybean ...

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Camelina seed transcriptome: a tool for meal and oil improvement and translational research

03-04-2013 | Huu T. Nguyen, Jillian E. Silva, Ram Podicheti, Jason Macrander, Wenyu Yang, Tara J. Nazarenus, Jeong‐Won Nam, Jan G ..., Plant Biotechnology Journal, 2013

Summary Camelina (Camelina sativa), a Brassicaceae oilseed, has received recent interest as a biofuel crop and production platform for industrial oils. Limiting wider production of camelina for these uses is the need to improve the quality and content of the seed protein‐rich meal and ...

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Transgenic rice seed synthesizing diverse flavonoids at high levels: a new platform for flavonoid production with associated health benefits

03-04-2013 | Yuko Ogo, Kenjiro Ozawa, Tsutomu Ishimaru, Tsugiya Murayama, Fumio Takaiwa, Plant Biotechnology Journal, 2013

Summary Flavonoids possess diverse health‐promoting benefits but are nearly absent from rice, because most of the genes encoding enzymes for flavonoid biosynthesis are not expressed in rice seeds. In the present study, a transgenic rice plant producing several classes of flavonoids in ...

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New insights into Chlamydomonas reinhardtii hydrogen production processes by combined microarray/RNA‐seq transcriptomics

03-04-2013 | Jörg Toepel, Maike Illmer‐Kephalides, Sebastian Jaenicke, Jasmin Straube, Patrick May, Alexander Goesmann, Olaf Kruse, Plant Biotechnology Journal, 2013

Summary Hydrogen production with Chlamydomonas reinhardtii induced by sulphur starvation is a multiphase process while the cell internal metabolism is completely remodelled. The first cellular response is characterized by induction of genes with regulatory functions, followed by a total ...

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