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176 Newest Publications in nature protocols
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Evaluation of the pathogenesis and treatment of Mycobacterium marinum infection in zebrafish
16-05-2013 | Kevin Takaki; J Muse Davis; Kathryn Winglee; Lalita Ramakrishnan, Nature Protocols, 2013
Nature Protocols 8, 1114 (2013). doi:10.1038/nprot.2013.068 Authors: Kevin Takaki, J Muse Davis, Kathryn Winglee & Lalita Ramakrishnan Mycobacterium marinum–infected zebrafish are used to study tuberculosis pathogenesis, as well as for antitubercular drug discovery. The ...
Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood
09-05-2013 | Giulietta Saletti; Nicolas Çuburu; Jae Seung Yang; Ayan Dey; Cecil Czerkinsky, Nature Protocols, 2013
Nature Protocols 8, 1073 (2013). doi:10.1038/nprot.2013.058 Authors: Giulietta Saletti, Nicolas Çuburu, Jae Seung Yang, Ayan Dey & Cecil Czerkinsky The enzyme-linked immunospot (ELISPOT) assay was originally developed to enumerate antigen-specific antibody-secreting cells ...
Construction and use of a microfluidic dissection platform for long-term imaging of cellular processes in budding yeast
02-05-2013 | Daphne H E W Huberts; Sung Sik Lee; Javier Gonzáles; Georges E Janssens; Ima Avalos Vizcarra; Matthias Heinemann, Nature Protocols, 2013
Nature Protocols 8, 1019 (2013). doi:10.1038/nprot.2013.060 Authors: Daphne H E W Huberts, Sung Sik Lee, Javier Gonzáles, Georges E Janssens, Ima Avalos Vizcarra & Matthias Heinemann This protocol describes the production and operation of a microfluidic dissection platform ...
Small-scale isolation of synaptic vesicles from mammalian brain
25-04-2013 | Saheeb Ahmed; Matthew Holt; Dietmar Riedel; Reinhard Jahn, Nature Protocols, 2013
Nature Protocols 8, 998 (2013). doi:10.1038/nprot.2013.053 Authors: Saheeb Ahmed, Matthew Holt, Dietmar Riedel & Reinhard Jahn Synaptic vesicles (SVs) are essential organelles that participate in the release of neurotransmitters from a neuron. Biochemical analysis of ...
Studying task-related activity of individual neurons in the human brain
18-04-2013 | Shaun R Patel; Sameer A Sheth; Clarissa Martinez-Rubio; Matthew K Mian; Wael F Asaad; Jason L Gerrard; Churl-Su Kwon ..., Nature Protocols, 2013
Nature Protocols 8, 949 (2013). doi:10.1038/nprot.2013.050 Authors: Shaun R Patel, Sameer A Sheth, Clarissa Martinez-Rubio, Matthew K Mian, Wael F Asaad, Jason L Gerrard, Churl-Su Kwon, Darin D Dougherty, Alice W Flaherty, Benjamin D Greenberg, John T Gale, Ziv M Williams & ...
Collecting, rearing, spawning and inducing regeneration of the starlet sea anemone, Nematostella vectensis
11-04-2013 | Derek J Stefanik; Lauren E Friedman; John R Finnerty, Nature Protocols, 2013
Nature Protocols 8, 916 (2013). doi:10.1038/nprot.2013.044 Authors: Derek J Stefanik, Lauren E Friedman & John R Finnerty Over the past 20 years, the starlet sea anemone, Nematostella vectensis, a small estuarine animal, has emerged as a powerful model system for field and ...
Genome-wide mapping of RNA structure using nuclease digestion and high-throughput sequencing
04-04-2013 | Yue Wan; Kun Qu; Zhengqing Ouyang; Howard Y Chang, Nature Protocols, 2013
Nature Protocols 8, 849 (2013). doi:10.1038/nprot.2013.045 Authors: Yue Wan, Kun Qu, Zhengqing Ouyang & Howard Y Chang RNA structure is important for RNA function and regulation, and there is growing interest in determining the RNA structure of many transcripts. Here we ...
Qualitative and quantitative metabolomic investigation of single neurons by capillary electrophoresis electrospray ionization mass spectrometry
28-03-2013 | Peter Nemes; Stanislav S Rubakhin; Jordan T Aerts; Jonathan V Sweedler, Nature Protocols, 2013
Nature Protocols 8, 783 (2013). doi:10.1038/nprot.2013.035 Authors: Peter Nemes, Stanislav S Rubakhin, Jordan T Aerts & Jonathan V Sweedler Single-cell mass spectrometry (MS) empowers metabolomic investigations by decreasing analytical dimensions to the size of individual ...
A rapid and reproducible assay for modeling myelination by oligodendrocytes using engineered nanofibers
21-03-2013 | Seonok Lee; S Y Christin Chong; Samuel J Tuck; Joseph M Corey; Jonah R Chan, Nature Protocols, 2013
Nature Protocols 8, 771 (2013). doi:10.1038/nprot.2013.039 Authors: Seonok Lee, S Y Christin Chong, Samuel J Tuck, Joseph M Corey & Jonah R Chan Current methods for studying oligodendrocyte myelination using primary neurons are limited by the time, cost and reproducibility ...
Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA
14-03-2013 | Marie-Theres Gansauge; Matthias Meyer, Nature Protocols, 2013
Nature Protocols 8, 737 (2013). doi:10.1038/nprot.2013.038 Authors: Marie-Theres Gansauge & Matthias Meyer This protocol describes a method for converting short single-stranded and double-stranded DNA into libraries compatible with high-throughput sequencing using Illumina ...
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