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745 Newest Publications in biotechnology letters
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Biocatalytic characterization of a short-chain alcohol dehydrogenase with broad substrate specificity from thermophilic Carboxydothermus hydrogenoformans
19-11-2012 | Shuo Zhou, Shun-Cheng Zhang, Dun-Yue Lai, Shuang-Ling Zhang, Zhen-Ming Chen, Biotechnology Letters, 2012
The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over ...
Metabolic engineering of morphinan alkaloids by over-expression of codeinone reductase in transgenic hairy roots of Papaver bracteatum, the Iranian poppy
19-11-2012 | Ali Sharafi, Haleh Hashemi Sohi, Amir Mousavi, Pejman Azadi, Bahman Hosseini Khalifani, Khadijeh Razavi, Biotechnology Letters, 2012
Papaver bracteatum has a high content of thebaine. It is used as an alternative to P. somniferum for the production of benzylisoquinoline alkaloid. Papaver bracteatum was genetically engineered to over-express codeinone reductase gene in hairy root cultures. Transcript level of the codeinone ...
Efficient biological process characterization by definitive-screening designs: the formaldehyde treatment of a therapeutic protein as a case study
19-11-2012 | Axel Erler, Nuria de Mas, Philip Ramsey, Grant Henderson, Biotechnology Letters, 2012
As part of the process-characterization campaign of a candidate vaccine product, a recently developed class of three-level designs—definitive-screening designs—was employed to select a quadratic model that describes the effect of six input process parameters, including protein concentration, ...
Accelerated myotube formation using bioprinting technology for biosensor applications
19-11-2012 | Xiaofeng Cui, Guifang Gao, Yongjun Qiu, Biotechnology Letters, 2012
Muscle-powered, biological, microelectro-mechanical system is promising for actuator and biosensor applications. Functional conjugation between the cells, tissues, and biomolecules to the microdevice is crucial for this application. Bioprinting as an enabling technology possesses the ...
Dimensionality reduction for microarray data using local mean based discriminant analysis
19-11-2012 | Yan Cui, Chun-Hou Zheng, Jian Yang, Biotechnology Letters, 2012
A new method is proposed for finding a low dimensional subspace of high dimensional microarray data. We developed a new criterion for constructing the weight matrix by using local neighborhood information to discover the intrinsic discriminant structure in the data. Also this approach applies ...
Novel technique for internal structure and elemental distribution analyses of granular sludge from reactors for wastewater treatment
19-11-2012 | Xiaolei Cao, Hongbin Cao, Yuxing Sheng, Haixia You, Yi Zhang, Biotechnology Letters, 2012
A novel technique for internal structure and elemental distribution analyses of granular sludge is presented. Sludge samples were freeze-dried and embedded in epoxy resin to form a module, which were then ground and polished to obtain sequential cross-sections. The cross-sections were ...
Endogenous signal peptides efficiently mediate the secretion of recombinant proteins in Pichia pastoris
19-11-2012 | Shuli Liang, Cheng Li, Yanrui Ye, Ying Lin, Biotechnology Letters, 2012
By predicting the potential signal peptides from proteins that are naturally secreted by Pichia pastoris, we identified three possible endogenous signal peptides: Scw, Dse and Exg. We compared their capability to mediate the secretion of enhanced green fluorescent protein (EGFP) and Candida ...
Comparative study of in vitro expansion of bone marrow-derived mesenchymal stem cells
19-11-2012 | Stefan Peter, Andy M. Scutt, Phillip C. Wright, Catherine A. Biggs, Biotechnology Letters, 2012
Bone marrow-derived mesenchymal stem cells (BMD-MSCs) are of great interest for tissue engineering, but require expansion before they can be used for therapeutic applications. We compared three different culture techniques for their potential for large scale expansion of rat BMD-MSCs, i.e. ...
Identification of a novel fosfomycin-resistant UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from a soil metagenome
10-11-2012 | Gong Cheng, Yongfei Hu, Na Lu, Jing Li, Zhiyun Wang, Quanze Chen, Baoli Zhu, Biotechnology Letters, 2012
A soil metagenomic library was constructed and screened for clones that conferred fosfomycin resistance. A novel protein with 46 % identity to UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from Desulfuromonas acetoxidans DSM 684 (GenBank accession number: ZP_01311756) was identified. ...
Quantitative evaluation of Candia antarctica lipase B displayed on the cell surface of a Pichia pastoris based on an FS anchor system
10-11-2012 | Xing-xiang Liang, Bei-bei Wang, Yu-fei Sun, Ying Lin, Shuang-yan Han, Sui-ping Zheng, Tang-bing Cui, Biotechnology Letters, 2012
A new approach is described to quantify the number of enzyme molecules, such as Candia antarctica lipase B, that are displayed on the cell surface of Pichia pastoris. Enhanced green fluorescent protein (EGFP) and Candida antarctica lipase B (CALB) were fused and displayed on the surface of P. ...
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