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428 Newest Publications in biotechnology and bioprocess engineering

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Ionic liquids as selectors for controlling the crystallization nucleation of hen egg white lysozyme

23-10-2012 | Zhanzhong Wang, Qian Wang, Leping Dang, Biotechnology and Bioprocess Engineering, 2012

Ionic liquids (ILs) have biomaterial applications and are used for protein crystallization. The effect of two imidazolium-based ILs, 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) and 1,3-dimethylimidazolium iodide ([dmim]I), on the nucleation kinetics of lysozyme was investigated by ...

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Ethanol production by In situ xylose isomerization using recombinant Escherichia coli and fermentation using conventional Saccharomyces cerevisiae

23-10-2012 | Zhibin Liu, Jin Chuan Wu, Biotechnology and Bioprocess Engineering, 2012

Xylose isomerase from Geobacillus kaustophilus HTA426 was functionally expressed in Escherichia coli BL21 (DE3) and the recombinant E. coli cells were used together with conventional Saccharomyces cerevisiae to produce ethanol from xylose by simultaneous xylose isomerisation and fermentation. ...

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Immobilization of steapsin lipase on macroporous immobead-350 for biodiesel production in solvent free system

23-10-2012 | Kishor P. Dhake, Kushal D. Bhatte, Yogesh S. Wagh, Rekha S. Singhal, Bhalchandra M. Bhanage, Biotechnology and Bioprocess Engineering, 2012

Commercially available steapsin lipase was immobilized on macroporous polymer beads (IB-350) and further investigated for biodiesel production under solvent free conditions. The fatty acid methyl ester (biodiesel) synthesis was carried out by the methanolysis of fresh and used cooking ...

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Genotyping of the porcine ryanodine receptor 1 (RYR1) and estrogen receptor 1 (ESR1) genes by high resolution melting (HRM) approach

23-10-2012 | Jideng Ma, Mingzhou Li, Huiyu Wang, Xuewei Li, Biotechnology and Bioprocess Engineering, 2012

During the last decade, DNA mutations in the porcine ryanodine receptor 1 gene (RYR1, C1843T) and the estrogen receptor 1 gene (ESR1, T1665G), have been widely used in marker-assisted selection (MAS) for the pig industry. These 2 well-characterized SNPs in RYR1 and ESR1 are responsible for ...

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Evaluation of a high surface area fractional precipitation process for the purification of paclitaxel from Taxus chinensis

23-10-2012 | Min-Gyeong Han, Jin-Hyun Kim, Biotechnology and Bioprocess Engineering, 2012

In this study, we evaluated a high surface area fractional precipitation process to achieve the effective purification of paclitaxel, an anticancer agent, from plant cell cultures. Fractional precipitation experiments were performed by increasing the surface area per working volume (S/V) to ...

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Process design and evaluation of value-added chemicals production from biomass

23-10-2012 | A. -Ra Go, Jae Wook Ko, Sang Jun Lee, Seung Wook Kim, Sung Ok Han, Jinwon Lee, Han Min Woo, Youngsoon Um, Jaewook Na ..., Biotechnology and Bioprocess Engineering, 2012

Three different biodiesel production processes were simulated using the SuperPro Designer program. The process for producing biodiesel from soybean oil and methanol was designed using commercial chemical catalysts. This chemical process was compared with the biological process catalyzed by ...

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The effects of N+ ion implantation mutagenesis on the laccase production of Ceriporiopsis subvermispora

23-10-2012 | Chao Wang, Linlin Zhang, Hong Xu, Biotechnology and Bioprocess Engineering, 2012

In order to obtain genetically stable, high-yield, laccase-producing strains, Ceriporiopsis subvermispora was induced by N+ ion implantation and subcultured. The results revealed that, with energy of 30 keV and a dose of 80×1014 ions/cm2, a relatively high increase in mutations and positive ...

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The production of bioflocculants by Bacillus licheniformis using molasses and its application in the sugarcane industry

23-10-2012 | Xiaoling Zhuang, Yuanpeng Wang, Qingbiao Li, Shan Yan, Ning He, Biotechnology and Bioprocess Engineering, 2012

A bioflocculant produced by B. licheniformis was investigated with regard to a low-cost culture medium and its industrial application. Molasses replaced sucrose as the sole carbon source in bioflocculant fermentation. The optimum low-cost culture medium was determined to be composed of 20 g/L ...

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Isolation and antioxidant activity evaluation of two new phthalate derivatives from seahorse, Hippocampus Kuda Bleeler

23-10-2012 | Zhong-Ji Qian, Kyong-Hwa Kang, Se-Kwon Kim, Biotechnology and Bioprocess Engineering, 2012

Seahorse (Hippocampus Kuda Bleeler) has been used as traditional medicine for thousands of years, in Eastern Asia. In this study of the methanol extract of fresh Hippocampus Kuda, the new compounds 2-ethyldecyl 2-ethylundecyl phthalate (1), 2, 12-diethyl-11-methylhexadecyl ...

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Production of salmosin, a snake venom-derived disintegrin, in recombinant Pichia pastoris using high cell density fed-batch fermentation

23-10-2012 | Myung-Ji Seo, Hak-Jong Choi, Kwang-Hoe Chung, Yu-Ryang Pyun, Biotechnology and Bioprocess Engineering, 2012

Salmosin, a snake venom-derived disintegrin, was successfully expressed in the methylotrophic yeast Pichia pastoris and secreted into the culture supernatant, as a 6 kDa protein. High-cell density fermentation of recombinant P. pastoris was optimized for the mass production of salmosin. In a ...

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