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304 Newest Publications in biopolymers
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Extrusion based rapid prototyping technique ‐ An advanced platform for tissue engineering scaffold fabrication
09-08-2011 | M. Enamul Hoque; Y. Leng Chuan; Ian Pashby, Biopolymers, 2011
Abstract Advances in scaffold design and fabrication technology have brought the tissue engineering field stepping into a new era. Conventional techniques used to develop scaffolds inherit limitations, such as lack of control over the pore morphology and architecture as well as ...
Structure of micelle‐bound adrenomedullin, a first step towards the analysis of its interactions with receptors and small molecules
09-08-2011 | Pérez‐Castells, Javier; Martín‐Santamaría, Sonsoles; Nieto, Lidia; Ramos, Ana; Ma ..., Biopolymers, 2011
Abstract Adrenomedullin (AM) is a regulatory peptide which plays many physiological roles including vasodilatation, bronchodilatation, hormone secretion regulation, growth, apoptosis, angiogenesis, and antimicrobial activities, among others. These regulatory activities make AM a relevant ...
Characterizing flexible and intrinsically unstructured biological macromolecules by SAS using the Porod‐Debye law
01-08-2011 | Rambo, Robert P.; Tainer, John A., Biopolymers, 2011
Abstract Unstructured proteins, RNA or DNA components provide functionally important flexibility that is key to many macromolecular assemblies throughout cell biology. As objective, quantitative experimental measures of flexibility and disorder in solution are limited, small angle ...
Invited review: Probing the structures of muscle regulatory proteins using small‐angle solution scattering
01-08-2011 | Lu, Yanling; Jeffries, Cy M.; Trewhella, Jill, Biopolymers, 2011
Abstract Small‐angle X‐ray and neutron scattering with contrast variation have made important contributions in advancing our understanding of muscle regulatory protein structures in the context of the dynamic molecular processes governing muscle action. The contributions of the scattering ...
Small angle X‐ray scattering as a complementary tool for high‐throughput structural studies
01-08-2011 | Grant, Thomas D.; Luft, Joseph R.; Wolfley, Jennifer R.; Tsuruta, Hiro; Martel, Anne; Montelione, Gaetano T.; Snell, ..., Biopolymers, 2011
Abstract Structural crystallography and nuclear magnetic resonance (NMR) spectroscopy are the predominant techniques for understanding the biological world on a molecular level. Crystallography is constrained by the ability to form a crystal that diffracts well and NMR is constrained to ...
X‐ray solution scattering studies of the structural diversity intrinsic to protein ensembles
01-08-2011 | Makowski, Lee; Gore, David; Mandava, Suneeta; Minh, David; Park, Sanghyun; Rodi, Diane J.; Fischetti, Robert F., Biopolymers, 2011
Abstract It is becoming increasingly clear that characterization of the protein ensemble—the collection of all conformations of which the protein is capable—will be a critical step in developing a full understanding of the linkage between structure, dynamics, and function. X‐ray solution ...
Minireview: Structural insights into early folding events using continuous‐flow time‐resolved small‐angle X‐ray scattering
01-08-2011 | Kathuria, Sagar V.; Guo, Liang; Graceffa, Rita; Barrea, Raul; Nobrega, R. Paul; Matthews, C. Robert; Irving, Thomas ..., Biopolymers, 2011
Abstract Small‐angle X‐ray scattering (SAXS) is a powerful method for obtaining quantitative structural information on the size and shape of proteins, and it is increasingly used in kinetic studies of folding and association reactions. In this minireview, we discuss recent developments in ...
Time resolved SAXS and RNA folding
01-08-2011 | Pollack, Lois, Biopolymers, 2011
Abstract Small angle X‐ray scattering provides low resolution structural information about macromolecules in solution. When coupled with rapid mixing methods, SAXS reports time‐dependent conformational changes of RNA induced by the addition of Mg2+ to trigger folding. Thus time‐resolved ...
Functionalization of a protein surface with per‐O‐methylated β‐cyclodextrin
21-07-2011 | Kitagishi, Hiroaki; Kashiwa, Kazuya; Kano, Koji, Biopolymers, 2011
Abstract Per‐O‐methylated β‐cyclodextrin (CD) bearing an iodoacetamide group at the 6‐position was synthesized to functionalize protein surfaces. Bovine serum albumin (BSA) was quantitatively modified with the CD derivative by the SN2 reaction of iodoacetamide with a cysteine residue ...
Molecular model of human heparanase with proposed binding mode of a heparan sulfate oligosaccharide and catalytic amino acids
21-07-2011 | Sapay, Nicolas; Cabannes, Éric; Petitou, Maurice; Imberty, Anne, Biopolymers, 2011
Abstract Heparan sulfate is abundantly present in the extracellular matrix. As other glycosaminoglycans, it is synthesized in the Golgi apparatus and then exposed on the cell surface. The glucuronidase activity of human heparanase plays a major role in the structural remodeling of the ...
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