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1,664 Newest Publications in applied microbiology and biotechnology

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DNA restriction-modification systems in the ethanologen, Zymomonas mobilis ZM4

20-10-2010 | Aidan L. Kerr, Young Jae Jeon, Charles J. Svenson, Peter L. Rogers, Brett A. Neilan, Applied Microbiology and Biotechnology, 2010

To better understand the DNA restriction-modification (R-M) systems for more amenable strain development of the alternative industrial ethanologen, Zymomonas mobilis, three gene knockout mutants were constructed. The gene knockout mutants were tested for their DNA restriction activities by ...

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Elimination of carbon catabolite repression in Klebsiella oxytoca for efficient 2,3-butanediol production from glucose–xylose mixtures

20-10-2010 | Xiao-Jun Ji, Zhi-Kui Nie, He Huang, Lu-Jing Ren, Chao Peng, Ping-Kai Ouyang, Applied Microbiology and Biotechnology, 2010

Microbial preference for glucose implies incomplete and/or slow utilization of lignocellulose hydrolysates, which is caused by the regulatory mechanism named carbon catabolite repression (CCR). In this study, a 2,3-butanediol (2,3-BD) producing Klebsiella oxytoca strain was engineered to ...

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RNAi as a potential tool for biotechnological applications in fungi

16-10-2010 | Tomer M. Salame, Carmit Ziv, Yitzhak Hadar, Oded Yarden, Applied Microbiology and Biotechnology, 2010

RNA interference (RNAi) is a post-transcriptional gene-silencing (PTGS) phenomenon in which double-stranded RNA (dsRNA) triggers the degradation of homologous mRNA species, thereby reducing gene expression. In fungi, the use of RNAi as a tool for reverse genetics, aimed at modification of ...

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Ionic liquids for two-phase systems and their application for purification, extraction and biocatalysis

16-10-2010 | Sebastian Oppermann, Florian Stein, Udo Kragl, Applied Microbiology and Biotechnology, 2010

The development of biotechnological processes using novel two-phase systems based on molten salts known as ionic liquids (ILs) got into the focus of interest. Many new approaches for the beneficial application of the interesting solvent have been published over the last years. ILs bring ...

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Genetic analysis around aminoalcohol dehydrogenase gene of Rhodococcus erythropolis MAK154: a putative GntR transcription factor in transcriptional regulation

16-10-2010 | Nobuyuki Urano, Michihiko Kataoka, Takeru Ishige, Shinji Kita, Keiji Sakamoto, Sakayu Shimizu, Applied Microbiology and Biotechnology, 2010

NADP+-dependent aminoalcohol dehydrogenase (AADH) of Rhodococcus erythropolis MAK154 catalyzes the reduction of (S)-1-phenyl-1-keto-2-methylaminopropane ((S)-MAK) to d-pseudoephedrine, which is used as a pharmaceutical. AADH is suggested to participate in aminoalcohol or aminoketone ...

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Isolation and characterization of endophytic bacterium LRE07 from cadmium hyperaccumulator Solanum nigrum L. and its potential for remediation

16-10-2010 | Shenglian Luo, Yong Wan, Xiao Xiao, Hanjun Guo, Liang Chen, Qiang Xi, Guangming Zeng, Chengbin Liu, Jueliang Chen, Applied Microbiology and Biotechnology, 2010

Valuable endophytic strains facilitating plants growth and detoxification of heavy metals are required because the application of plant–endophyte symbiotic system is a promising potential technique to improve efficiency of phytoremediation. In this study, endophytic bacterium LRE07 was ...

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Polyoxometalate/laccase-mediated oxidative polymerization of catechol for textile dyeing

16-10-2010 | Suyeon Kim, Carla Silva, Dmitry V. Evtuguin, José A. F. Gamelas, Artur Cavaco-Paulo, Applied Microbiology and Biotechnology, 2010

The synergistic effect between polyoxometalates (POMs), namely K5[SiW11VVO40]·11H2O and H5[PMo10VV2O40]·13H2O and laccase from ascomycete Myceliophthora thermophila has been employed for the first time in oxidative polymerization of catechol. Such a laccase-mediator system allowed the ...

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Spatial distribution and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in mangrove sediments

16-10-2010 | Meng Li, Huiluo Cao, Yiguo Hong, Ji-Dong Gu, Applied Microbiology and Biotechnology, 2010

We investigated the diversity, spatial distribution, and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediment samples of different depths collected from a transect with different distances to mangrove forest in the territories of Hong Kong. Both the ...

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Antimicrobial polymers: mechanism of action, factors of activity, and applications

16-10-2010 | Larisa Timofeeva, Natalia Kleshcheva, Applied Microbiology and Biotechnology, 2010

Complex epidemiological situation, nosocomial infections, microbial contamination, and infection risks in hospital and dental equipment have led to an ever-growing need for prevention of microbial infection in these various areas. Macromolecular systems, due to their properties, allow one to ...

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Elaboration of antibiofilm materials by chemical grafting of an antimicrobial peptide

16-10-2010 | Jean-Fabrice Yala, Pascal Thebault, Arnaud Héquet, Vincent Humblot, Claire-Marie Pradier, Jean-Marc Berjeaud, Applied Microbiology and Biotechnology, 2010

A peptide antibiotic, gramicidin A, was covalently bound to cystamine self-assembled monolayers on gold surfaces. Each step of the surface functionalization was characterized by polarization modulation infrared reflection absorption spectroscopy and X-ray photoelectron spectroscopy. The ...

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