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893 Newest Publications in applied biochemistry and biotechnology
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Actinobacterial Peroxidases: an Unexplored Resource for Biocatalysis
29-01-2011 | Marilize le Roes-Hill, Nuraan Khan, Stephanie Gail Burton, Applied Biochemistry and Biotechnology, 2011
Peroxidases are redox enzymes that can be found in all forms of life where they play diverse roles. It is therefore not surprising that they can also be applied in a wide range of industrial applications. Peroxidases have been extensively studied with particular emphasis on those isolated ...
Green Pigment from Bacillus cereus M116 (MTCC 5521): Production Parameters and Antibacterial Activity
29-01-2011 | Debopam Banerjee, Sandipan Chatterjee, U. C. Banerjee, Arun K. Guha, Lalitagauri Ray, Applied Biochemistry and Biotechnology, 2011
A bacterial strain, Bacillus cereus M116 (MTCC 5521), isolated and identified in our laboratory produces a green pigment when grown in nutrient broth at stationary condition. Optimum fermentation parameters for maximum pigment production are pH 7.0, temperature 30°C, time of incubation 72 h ...
Propyl Gallate Synthesis Using Acidophilic Tannase and Simultaneous Production of Tannase and Gallic Acid by Marine Aspergillus awamori BTMFW032
28-01-2011 | P. S. Beena, Soorej M. Basheer, Sarita G. Bhat, Ali H. Bahkali, M. Chandrasekaran, Applied Biochemistry and Biotechnology, 2011
Marine Aspergillus awamori BTMFW032, recently reported by us, produce acidophilic tannase as extracellular enzyme. Here, we report the application of this enzyme for synthesis of propyl gallate by direct transesterification of tannic acid and in tea cream solubilisation besides the ...
Effect of the Conformational Stability of the CH2 Domain on the Aggregation and Peptide Cleavage of a Humanized IgG
28-01-2011 | Daisuke Kameoka, Tadashi Ueda, Taiji Imoto, Applied Biochemistry and Biotechnology, 2011
To examine the effect of the conformational stability of the CH2 domain on aggregation and peptide cleavage of a humanized IgG1, we carried out size exclusion chromatography and sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses of incubated sample solutions. By comparing the ...
Construction, Expression, and Characterization of a Single-Chain Variable Fragment Antibody Against 2,4-Dichlorophenoxyacetic Acid in the Hemolymph of Silkworm Larvae
28-01-2011 | Seiichi Sakamoto, Benyakan Pongkitwitoon, Seiko Nakamura, Kaori Sasaki-Tabata, Yusuke Tanizaki, Katsumi Maenaka, Hir ..., Applied Biochemistry and Biotechnology, 2011
A single-chain variable fragment antibody against herbicide, 2,4-dichlorophenoxyacetic acid (2,4-D-scFv) has been successfully expressed in the hemolymph of silkworm larvae using a rapid Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid DNA system. Variable heavy- and light-chain domains were ...
Two-Stage Fractionation of Corn Stover Using Aqueous Ammonia and Hot Water
27-01-2011 | Chang Geun Yoo, Chi-Woo Lee, Tae Hyun Kim, Applied Biochemistry and Biotechnology, 2011
Hot water and aqueous ammonia fractionation of corn stover were used to separate hemicellulose and lignin and improve enzymatic digestibility of cellulose. A two-stage approach was used: The first stage was designed to recover soluble lignin using aqueous ammonia at low temperature, while the ...
Cellulase Production by Pink Pigmented Facultative Methylotrophic Strains (PPFMs)
27-01-2011 | Shanmugam Jayashree, Rajendran Lalitha, Ponnusamy Vadivukkarasi, Yuko Kato, Sundaram Seshadri, Applied Biochemistry and Biotechnology, 2011
Pink pigmented facultative methylotrophs (PPFM) isolated from water samples of Cooum and Adyar rivers in Chennai and soil samples of forests located in various districts of Tamil Nadu, India were screened for cellulase production using carboxymethylcellulose agar (CMC agar) medium. The ...
Production of Astaxanthin from Cellulosic Biomass Sugars by Mutants of the Yeast Phaffia rhodozyma
27-01-2011 | Justin Montanti, Nhuan P. Nghiem, David B. Johnston, Applied Biochemistry and Biotechnology, 2011
Astaxanthin is a potential high-value coproduct in an ethanol biorefinery. Three mutant strains of the astaxanthin-producing yeast Phaffia rhodozyma, which were derived from the parent strain ATCC 24202 (UCD 67-210) and designated JTM166, JTM185, and SSM19, were tested for their capability of ...
Development and Characterization of an Environmentally Friendly Process Sequence (Autohydrolysis and Organosolv) for Wheat Straw Delignification
27-01-2011 | Héctor A. Ruiz, Denise S. Ruzene, Daniel P. Silva, Fernando F. Macieira da Silva, António A. Vicente, José A. Teixeira, Applied Biochemistry and Biotechnology, 2011
The present work describes the delignification of wheat straw through an environmentally friendly process resulting from sequential application of autohydrolysis and organosolv processes. Wheat straw autohydrolysis was performed at 180°C during 30 min with a liquid–solid ratio of 10 (v/w); ...
Modulation of Triterpene Saponin Production: In Vitro Cultures, Elicitation, and Metabolic Engineering
26-01-2011 | Ellen Lambert, Ahmad Faizal, Danny Geelen, Applied Biochemistry and Biotechnology, 2011
Saponins are secondary metabolites that are widely distributed in the plant kingdom and are often the active components in medicinal herbs. Hence, saponins have a potential for the pharmaceutical industry as antibacterial, virucidal, anti-inflammatory, and anti-leishmanial drugs. However, ...
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