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893 Newest Publications in applied biochemistry and biotechnology
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Isolation, Identification and Optimization of a New Extracellular Lipase Producing Strain of Rhizopus sp.
08-02-2011 | Jayshree B. Kantak, Aditi V. Bagade, Siddharth A. Mahajan, Shrikant P. Pawar, Yogesh S. Shouche, Asmita Ashutosh Pra ..., Applied Biochemistry and Biotechnology, 2011
A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S–ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, ...
Ethanol Production from Cashew Apple Bagasse: Improvement of Enzymatic Hydrolysis by Microwave-Assisted Alkali Pretreatment
08-02-2011 | Tigressa Helena Soares Rodrigues, Maria Valderez Ponte Rocha, Gorete Ribeiro de Macedo, Luciana R. B. Gonçalves, Applied Biochemistry and Biotechnology, 2011
In this work, the potential of microwave-assisted alkali pretreatment in order to improve the rupture of the recalcitrant structures of the cashew able bagasse (CAB), lignocellulosic by-product in Brazil with no commercial value, is obtained from cashew apple process to juice production, was ...
Citrate Stimulates Oligosaccharide Synthesis in Metabolically Engineered Agrobacterium sp.
08-02-2011 | Anne M. Ruffing, Rachel Ruizhen Chen, Applied Biochemistry and Biotechnology, 2011
Agrobacterium sp. ATCC 31749 was previously shown to be an advantageous host for oligosaccharide production. Unexpectedly, the addition of citrate to the oligosaccharide synthesis reaction resulted in up to a sixfold improvement in the production N-aceytl-lactosamine, a disaccharide. The ...
Protein Refolding by N-Alkylpyridinium and N-Alkyl-N-methylpyrrolidinium Ionic Liquids
08-02-2011 | Etsushi Yamamoto, Satoshi Yamaguchi, Teruyuki Nagamune, Applied Biochemistry and Biotechnology, 2011
An important property of ionic liquids consisting of cations and anions is that the chemical structures can be easily tuned. To expand the repertoire of effective ionic liquid-based refolding additives, we focused on this tunable property and investigated the effects of new candidates such as ...
Purification and Properties of a Psychrotrophic Trichoderma sp. Xylanase and its Gene Sequence
08-02-2011 | Peng Zhou, Huifang Zhu, Qiaojuan Yan, Priti Katrolia, Zhengqiang Jiang, Applied Biochemistry and Biotechnology, 2011
A psychrotrophic fungus identified as Trichoderma sp. SC9 produced 36.7 U/ml of xylanase when grown on a medium containing corncob xylan at 20 °C for 6 days. The xylanase was purified 37-fold with a recovery yield of 8.2%. The purified xylanase appeared as a single protein band on SDS-PAGE ...
Biosynthesis and Characterization of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) Terpolymer with Various Monomer Compositions by Cupriavidus sp. USMAA2-4
08-02-2011 | Hema Ramachandran, Nurhezreen Md Iqbal, Coswald Stephen Sipaut, Amirul Al-Ashraf Abdullah, Applied Biochemistry and Biotechnology, 2011
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-4-hydroxybutyrate) [P(3HB-co-3HV-co-4HB)] terpolymer was produced using Cupriavidus sp. USMAA2-4 via one-step cultivation process through combination of various carbon sources such as 1,4-butanediol or γ-butyrolactone with either 1-pentanol, ...
Catalytic Performance of Corn Stover Hydrolysis by a New Isolate Penicillium sp. ECU0913 Producing both Cellulase and Xylanase
04-02-2011 | Qian-Qian Shi, Jie Sun, Hui-Lei Yu, Chun-Xiu Li, Jie Bao, Jian-He Xu, Applied Biochemistry and Biotechnology, 2011
A fungal strain, marked as ECU0913, producing high activities of both cellulase and xylanase was newly isolated from soil sample collected near decaying straw and identified as Penicillium sp. based on internal transcribed spacer sequence homology. The cultivation of this fungus produced both ...
Determination of Methyl Parathion in Water and Its Removal on Zirconia Using Optical Enzyme Assay
01-02-2011 | Kanchanmala Deshpande, Rupesh K. Mishra, Sunil Bhand, Applied Biochemistry and Biotechnology, 2011
A simple, miniaturized microplate chemiluminescence assay for determination of methyl parathion (MP) was developed in 384-microwell plates. Zirconia (ZrO2) was added in microwell for adsorption of acetylcholinesterase (AChE). The developed assay is based on inhibition of AChE by MP. A good ...
Converting Carbohydrates Extracted from Marine Algae into Ethanol Using Various Ethanolic Escherichia coli Strains
01-02-2011 | Soojin Lee, Younghoon Oh, Donghyun Kim, Doyeon Kwon, Choulgyun Lee, Jinwon Lee, Applied Biochemistry and Biotechnology, 2011
Marine algae, which make up about 80% of the world’s living organisms, contain many energy sources, such as sugars and lipids. Therefore, the possibility of utilizing structural carbohydrates from marine algae for bioethanol production has been studied. In order to obtain monosaccharides, ...
Production of a Monoclonal Antibody by Simultaneous Immunization of Staphylococcal Enterotoxin A and B
01-02-2011 | Bin Liang, Yongxia Zhang, Aiping Liu, Youxiang Zhou, Fusheng Chen, Xiaohong Wang, Applied Biochemistry and Biotechnology, 2011
In this paper, a method of simultaneous immunizing BALB/c mice with staphylococcal enterotoxin (SE) A and B (SEA and SEB) to prepare a monoclonal antibody (3F2) for detecting both of SEA and SEB was developed. The results showed that antibody 3F2 had high titers against both SEA and SEB by ...
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